Human FoxP3 Antibody Summary
Accession # Q9BZS1
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FoxP3 by Western Blot. Western blot shows lysates of human CD4+CD25+T cells and human CD4+CD25-T cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3240) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FoxP3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
FoxP3 in Human Tonsil. FoxP3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 10 µg/mL Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3240) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of FoxP3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. FoxP3/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3240) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheIL-2promoter was detected by standard PCR.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.
Citations for Human FoxP3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma
Authors: R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan
Journal of personalized medicine, 2022;12(4):.
Sample Types: Whole Tissue
Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer
Authors: Y Gao, X Li, Z Shu, K Zhang, X Xue, W Li, Q Hao, Z Wang, W Zhang, S Wang, C Zeng, D Fan, W Zhang, Y Zhang, H Zhao, M Li, C Zhang
Cell Death Dis, 2018;9(4):416.
Sample Types: Whole Cells
Applications: Flow Cytometry
Human CD4+ HLA-G+ regulatory T cells are potent suppressors of graft-versus-host disease in vivo.
Authors: Pankratz S, Bittner S, Herrmann A, Schuhmann M, Ruck T, Meuth S, Wiendl H
FASEB J, 2014;28(8):3435-45.
Sample Types: Cell Lysates
Applications: Western Blot
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