Human FoxP3 Antibody

Catalog # Availability Size / Price Qty
AF3240
AF3240-SP
Detection of Human FoxP3 by Western Blot.
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Product Details
Citations (4)
FAQs
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Human FoxP3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxP3
Gln105-Lys200
Accession # Q9BZS1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FoxP3 antibody by Western Blot. View Larger

Detection of Human FoxP3 by Western Blot. Western blot shows lysates of human CD4+CD25+T cells and human CD4+CD25-T cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3240) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FoxP3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry FoxP3 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

FoxP3 in Human Tonsil. FoxP3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 10 µg/mL Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3240) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chromatin Immunoprecipitation (ChIP) Detection of FoxP3-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of FoxP3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. FoxP3/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3240) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheIL-2promoter was detected by standard PCR.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name
Forkhead Box P3
Entrez Gene IDs
50943 (Human); 20371 (Mouse)
Alternate Names
AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FoxP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked

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Citations for Human FoxP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
    Authors: Octavian Bucur, Feifei Fu, Mike Calderon, Geetha H. Mylvaganam, Ngoc L. Ly, Jimmy Day et al.
    Nature Protocols
  2. Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma
    Authors: R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan
    Journal of personalized medicine, 2022-04-13;12(4):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer
    Authors: Y Gao, X Li, Z Shu, K Zhang, X Xue, W Li, Q Hao, Z Wang, W Zhang, S Wang, C Zeng, D Fan, W Zhang, Y Zhang, H Zhao, M Li, C Zhang
    Cell Death Dis, 2018-04-01;9(4):416.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human CD4+ HLA-G+ regulatory T cells are potent suppressors of graft-versus-host disease in vivo.
    Authors: Pankratz S, Bittner S, Herrmann A, Schuhmann M, Ruck T, Meuth S, Wiendl H
    FASEB J, 2014-04-17;28(8):3435-45.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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