Human/Mouse/Rat GDF-8/Myostatin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF788
GDF‑8/Myostatin in Mouse Embryonic Lung.
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Citations (1)
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Human/Mouse/Rat GDF-8/Myostatin Biotinylated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GDF‑8/Myostatin in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human/mouse/rat GDF-11 is observed, 10% cross-reactivity with recombinant mouse (rm) GDF‑3 is observed, and less than 2% cross-reactivity with rmGDF-1, rmGDF-5, and rmGDF-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF‑8/Myostatin
Asp268-Ser376
Accession # O08689
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788-G8)
Immunohistochemistry
5-25 µg/mL
Immersion fixed frozen sections of mouse embryo (E13) and mouse embryonic lung, and immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GDF-8/Myostatin antibody in Mouse Embryonic Lung by Immunohistochemistry (IHC-Fr). View Larger

GDF‑8/Myostatin in Mouse Embryonic Lung. GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (lung) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry View Larger

Detection of GDF‑8/Myostatin in Mouse Embryo. GDF‑8/Myostatin was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF788) at 25 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF‑8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

References
  1. Storm, E.E. et al. (1994) Nature 368:639
  2. Sharma, M. et al. (1999) J. Cell Physiol. 180:1
  3. McPherron, A.C. et al. (1997) Nature 387:83
  4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306
  5. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902
Long Name
Growth Differentiation Factor 8
Entrez Gene IDs
2660 (Human); 17700 (Mouse)
Alternate Names
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin

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Citation for Human/Mouse/Rat GDF-8/Myostatin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural basis of specific inhibition of extracellular activation of pro- or latent myostatin by the monoclonal antibody SRK-015
    Authors: KB Dagbay, E Treece, FC Streich, JW Jackson, RR Faucette, A Nikiforov, SC Lin, CJ Boston, SB Nicholls, AD Capili, GJ Carven
    J. Biol. Chem., 2020;295(16):5404-5418.
    Species: Human
    Sample Types: Cell Culture Supernates

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