Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GDF-8/Myostatin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF‑8/Myostatin
Asp268-Ser376
Accession # O08689

Specificity

Detects human, mouse, and rat GDF‑8/Myostatin in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human/mouse/rat GDF-11 is observed, 10% cross-reactivity with recombinant mouse (rm) GDF‑3 is observed, and less than 2% cross-reactivity with rmGDF-1, rmGDF-5, and rmGDF-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody

GDF-8/Myostatin antibody in Mouse Embryonic Lung by Immunohistochemistry (IHC-Fr).

GDF‑8/Myostatin in Mouse Embryonic Lung.

GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (lung) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of GDF‑8/Myostatin in Mouse Embryo.

GDF‑8/Myostatin was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF788) at 25 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13) and mouse embryonic lung, and immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo.

Western Blot

0.1 µg/mL
Sample: Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788-G8)

Reviewed Applications

Read 1 review rated 1 using BAF788 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF‑8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

References

  1. Storm, E.E. et al. (1994) Nature 368:639
  2. Sharma, M. et al. (1999) J. Cell Physiol. 180:1
  3. McPherron, A.C. et al. (1997) Nature 387:83
  4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306
  5. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902

Long Name

Growth Differentiation Factor 8

Alternate Names

GDF8, MSLHP, MSTN, Myostatin

Entrez Gene IDs

2660 (Human); 17700 (Mouse)

Gene Symbol

MSTN

UniProt

O08689

Additional GDF-8/Myostatin Products

Product Documents for Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody

For research use only

Citations for Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody

Customer Reviews for Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody (1)

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  • Human/Mouse/Rat GDF-8/Myostatin Biotinylated Antibody
    Name: Sampath Kumar Anandan
    Application: Western Blot
    Sample Tested: Skeletal muscle tissue
    Species: Avian and Mouse
    Verified Customer | Posted 04/15/2024
    The test samples consisted of skeletal muscle tissue homogenates derived from mouse and avian muscle tissues. The homogenisation buffer contained protease inhibitor cocktail. The positive control used was 1μg of R&D systems recombinant myostatin/GDF8 (#788-G8). I had loaded 20μg of total protein for each of the avian and mouse samples, along with1μg of R&D systems recombinant myostatin/GDF8 (#788-G8) into a 4-15% gel. The electrophoresis was performed with MES running buffer at 90V constant current, and the proteins were subsequently blotted onto a 0.2μm nitrocellulose membrane. To check for transfer efficiency and protein integrity, i had stained the blot with Ponceau S and found that the transfer was efficient and the proteins were not degraded. Subsequently, i destained the blot with TBS-T and deionised water, and blocked the blots with 5% milk in TBS-T. Prior to incubating the membrane in primary antibody, i had cut the membrane such that one section contained all the animal tissue samples and the other section contained the recombinant GDF-8/myostatin and the western standard. The cut sections were incubated separately with identical concentrations of primary and secondary antibodies - R&D systems goat polyclonal GDF8 antibody (BAF788) at 2 µg/mL concentration, and R&D system anti-goat secondary antibody (HAF017) at 1:4000 dilution. The recombinant GDF-8/Myostatin positive control and the western standards produced clear and robust signals. However, the sections containing the samples derived from animal tissues (avian and mouse) failed to produce any discernible signal. I had repeated the same experiment above three times with increasing concentrations of primary antibody - 0.1, 0.25 and 2 µg/mL. However, all three experiments yielded the same result - strong signal for the recombinant GDF-8/Myostatin, but no visible signal detected with the avian and mouse tissue samples.
    Human/Mouse/Rat GDF‑8/Myostatin Biotinylated Antibody BAF788
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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