GRK7 Antibody (496831)

R&D Systems | Catalog # MAB4700

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 496831
View all GRK7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRK7
Lys342-Leu553
Accession # Q8WTQ7

Specificity

Detects human, mouse, and rat GRK7 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GRK7 Antibody (496831)

Detection of Human, Mouse, and Rat GRK7 antibody by Western Blot.

Detection of Human, Mouse, and Rat GRK7 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, BJAB human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat GRK7 Monoclonal Antibody (Catalog # MAB4700) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRK7 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for GRK7 Antibody (496831)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, BJAB human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRK7

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183-188 amino acids and a carboxyl-terminus of variable length. GRK1 and GRK7 are members of the rhodopsin kinase or visual GRK subfamily. GRK7 is expressed in the cone outer segments of the retina and is able to phosphorylate rhodopsin in a light-dependent manner. In addition to its retina expression, GRK7 has been detected in other tissues, including brain, olfactory bulb, liver, lung and pancreas.

Long Name

G protein-coupled Receptor Kinase 7

Alternate Names

GPRK7

Entrez Gene IDs

131890 (Human)

Gene Symbol

GRK7

UniProt

Q8WTQ7

Additional GRK7 Products

Product Documents for GRK7 Antibody (496831)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GRK7 Antibody (496831)

For research use only

Related Research Areas

Intracellular Kinases

Citations for GRK7 Antibody (496831)

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