Human/Mouse/Rat Jak1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4260G-100UG
Detection of Jak1 in Jurkat Human Cell Line by Flow Cytometry.
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Human/Mouse/Rat Jak1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Jak1.
Source
Monoclonal Rat IgG2B Clone # 413104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Jak1
Pro32-Phe286
Accession # P23458
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Jak1 in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of Jak1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # IC4260G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Jak1

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha.

Long Name
Janus Kinase 1
Entrez Gene IDs
3716 (Human)
Alternate Names
EC 2.7.10; Jak1; JAK-1; JAK1Atyrosine-protein kinase JAK1; JAK1B; Janus kinase 1EC 2.7.10.2; JTK3

Product Datasheets

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