Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat Lactate Dehydrogenase A/LDHA by Western Blot.
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Product Details
Citations (1)
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Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat Lactate Dehydrogenase A/LDHA in Western blots and detects human Lactate Dehydrogenase A/LDHA in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LDHB is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Lactate Dehydrogenase A/LDHA
Accession # P00338
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Lactate Dehydrogenase A/LDHA by Western Blot. View Larger

Detection of Human, Mouse, and Rat Lactate Dehydrogenase A/LDHA by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, mouse skeletal muscle tissue, and rat skeletal muscle tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7304) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lactate Dehydrogenase A/LDHA at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lactate Dehydrogenase A/LDHA

LDHA (lactate dehydrogenase A chain; also LDH-M and PIG-19) is a 34-36 kDa member of the LDH family of enzymes. It is a part of a cytoplasmic complex that is found principally in hepatocytes and skeletal muscle. The LDHA complex catalyzes the conversion of pyruvate to lactate, thereby generating NAD, the final step in anerobic glycolysis. This NAD is essential for the subsequent generation of ATP. Human LDHA is 332 amino acids (aa) in length. It contains an N-terminal coenzyme binding region, a central catalytic site, and at least nine utilized Lys acetylation and two Tyr phosphorylation sites. It also undergoes ISGylation where a 17 kDa product of the ISG15 gene is covalently attached to LDHA in a ubiquitin-like manner. LDHA forms tetramers composed of two dimers of varying composition. The tetramer may be homotetrameric (four monomers), or contain from one to three substitute LDHB monomers typically found in heart muscle. There are multiple splice variants. One possesses a five aa substitution for aa 237-332, a second contains a 45 aa substitution for aa 230-332, a third shows a deletion of aa 82-139, while a fourth utilizes an alternative start site 29 aa upstream of the standard site. Over aa 2-92, human LDHA shares 93% aa sequence identity with mouse LDHA.

Entrez Gene IDs
3939 (Human); 16828 (Mouse); 24533 (Rat)
Alternate Names
Cell proliferation-inducing gene 19 protein; EC 1.1.1; EC; GSD11; Lactate Dehydrogenase A; lactate dehydrogenase M; LDH muscle subunit; LDH1; LDHA; LDH-A; LDHM; LDH-M; L-lactate dehydrogenase A chain; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59

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Citation for Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lactate dehydrogenase inhibition synergizes with IL-21 to promote CD8+ T cell stemness and antitumor immunity
    Authors: D Hermans, S Gautam, JC García-Cañ, D Gromer, S Mitra, R Spolski, P Li, S Christense, R Nguyen, JX Lin, J Oh, N Du, S Veenbergen, J Fioravanti, R Ebina-Shib, C Bleck, LM Neckers, JD Rabinowitz, L Gattinoni, WJ Leonard
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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