Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LMW-PTP/ACP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LMW‑PTP/ACP1
Ala2-His158
Accession # P24666

Specificity

Detects human, mouse, and rat LMW‑PTP/ACP1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

Detection of Human, Mouse, and Rat LMW-PTP/ACP1 antibody by Western Blot.

Detection of Human, Mouse, and Rat LMW‑PTP/ACP1 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, human placenta tissue, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat LMW-PTP/ACP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5075) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, human placenta tissue, TF‑1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using AF5075 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LMW-PTP/ACP1

LMW-PTP (Low Molecular Weight Protein Tyrosine Phosphatase; also Red cell acid phosphatase 1/ACP1, HCPTP, and HAAP/Adipocyte acid phosphatase) is a 17‑18 kDa member of the LMWPTP family, PTP superfamily of enzymes. It is widely expressed, being found in cells diverse as B and T cells, endothelial cells and vascular smooth muscle cells. LMW-PTP dephosphorylates multiple substrates, often in a cell-dependent manner. Among the substrates for LMW-PTP include PDGR beta, EphA2 and beta -catenin. PMW-PTP is found intracellularly, generally associated with two cytoplasmic pools, one in the cytosol and the other accompaning the cytoskeleton. Human LMW-PTP is 158 amino acids (aa) in length. The catalytic region spans aa 9-154, and there are two acetylation sites at Ala2 and Lys156. Phosphorylation on Tyr132 and Tyr133 occurs on cytoskeleton-associated LMW-PTP, and has the effect of increasing its phosphatase activity. There are three alternative splice variants reported for LMW-PTP. One shows a 34 aa substitution for aa 41-74, a second shows a deletion of aa 41-74, while a third contains a 35 aa substitution for aa 78-158. The last two isoforms may function a negative regulators of LMW-PTP activity. Full-length LMW-PTP shares 86% aa sequence identity with mouse LMW-PTP.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Alternate Names

ACP1, HAAP, LMWPTP

Entrez Gene IDs

52 (Human); 11431 (Mouse); 24161 (Rat)

Gene Symbol

ACP1

UniProt

P24666

Additional LMW-PTP/ACP1 Products

Product Documents for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

Customer Reviews for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody (1)

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  • Human/Mouse/Rat LMW-PTP/ACP1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver and Adult kidney
    Species: Human, Mouse and Rat
    Verified Customer | Posted 02/24/2017
    Human/Mouse/Rat LMW‑PTP/ACP1 Antibody AF5075

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