|Detection of Human/Mouse/Rat MKK3/MKK6 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MKK3/MKK6 Monoclonal Antibody (Catalog # MAB2514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. Specific bands for MKK3 and MKK6 was detected at approximately 40 kDa and 38 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.|
Mitogen-activated protein kinase kinase 3 (MKK3), also known as MEK3, and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation by several upstream MAPK kinase kinases, including ASK1 and TAK1. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr.
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Showing Results 1 - 1 of 1
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided