Detection of Human, Mouse, and Rat MKK6 by Western Blot.
Western blot shows lysates of B16-F1 mouse melanoma cell line and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Human/Mouse/Rat MKK6 Monoclonal Antibody (Catalog # MAB1604) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. A specific band for MKK6 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MKK6 and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 by several upstream MAPK kinase kinases, including ASK1 and TAK1.
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