Human/Mouse/Rat MKK6 Antibody Summary
Accession # AAC50388
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat MKK6 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.05 µg/mL Rabbit Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1604) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human MKK6 (2 ng) was included. A specific band for MKK6 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human MKK6 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MKK6 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1604) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MKK6 at approximately 39 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).
- Goedert, M. et al. (1997) EMBO J. 16:3563.
- Moriguchi, T. et al. (1996) J. Biol. Chem. 271:26981.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
- Han, J. et al. (1996) J. Biol. Chem. 271:2886.
- Ichijo, H. et al. (1997) Science 275:90.
- Moriguchi, T. et al. (1996) J. Biol. Chem. 271:13675.
- Keesler, G. et al. (1998) Protein Expr. Purif. 14:221.
Product Specific NoticesThe purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This product is sold pursuant to a license from the University of Massachusetts, and the University of Massachusetts reserves all other rights to use these reagents. For information on purchasing a license for uses other than research, please contact the University of Massachusetts, Office of Technology Management, 333 South Street, Suite 400, Shrewsbury, Massachusetts, 01545.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse/Rat MKK6 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat MKK6 Antibody and earn rewards!
Have you used Human/Mouse/Rat MKK6 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image