Human/Mouse/Rat MKK6 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat MKK6 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MKK6
    Gly6-Asp334
    Accession # AAC50388
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.05 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat MKK6 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.05 µg/mL Rabbit Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1604) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human MKK6 (2 ng) was included. A specific band for MKK6 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKK6

Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).

  • References:
    1. Goedert, M. et al. (1997) EMBO J. 16:3563.
    2. Moriguchi, T. et al. (1996) J. Biol. Chem. 271:26981.
    3. Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
    4. Han, J. et al. (1996) J. Biol. Chem. 271:2886.
    5. Ichijo, H. et al. (1997) Science 275:90.
    6. Moriguchi, T. et al. (1996) J. Biol. Chem. 271:13675.
    7. Keesler, G. et al. (1998) Protein Expr. Purif. 14:221.
  • Long Name:
    Mitogen-activated Protein Kinase Kinase 6
  • Entrez Gene IDs:
    5608 (Human); 26399 (Mouse); 114495 (Rat)
  • Alternate Names:
    MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK6dual specificity mitogen-activated protein kinase kinase 6; MEK6; MEK6MAPKK 6; mitogen-activated protein kinase kinase 6; MKK6; MKK6MEK 6; PRKMK6; PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPKK3; SAPKK3EC 2.7.12.2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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