Human/Mouse/Rat N‑WASP Antibody

R&D Systems | Catalog # AF3854

R&D Systems
Discontinued Product
AF3854 has been discontinued. View all N-WASP products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all N-WASP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human N-WASP
Leu21-Asn270
Accession # O00401

Specificity

Detects human, mouse, and rat N-WASP in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat N‑WASP Antibody

Detection of Human, Mouse, and Rat N-WASP antibody by Western Blot.

Detection of Human, Mouse, and Rat N-WASP by Western Blot.

Western blot shows lysates of DU145 human prostate carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-WASP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

N-WASP antibody in Rat Brain Tissue by Immunohistochemistry (IHC-Fr).

N‑WASP in Rat Brain Tissue.

N-WASP was detected in perfusion fixed frozen sections of rat brain (cortex) tissue using Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat N‑WASP Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG-01M)

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex)

Western Blot

1 µg/mL
Sample: DU145 human prostate carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: N-WASP

N-WASP is a ubiquitously expressed member of the WASP family of proteins. The 65 kDa, 505 amino acid, N-WASP seems to play a central role as a node interconnecting various actin networks. Over the region used as immunogen, human N-WASP is greater than 98% identical to the corresponding mouse and canine protein sequences.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Entrez Gene IDs

8976 (Human); 73178 (Mouse); 296936 (Rat)

Gene Symbol

WASL

UniProt

O00401

Additional N-WASP Products

Product Documents for Human/Mouse/Rat N‑WASP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat N‑WASP Antibody

For research use only

Citations for Human/Mouse/Rat N‑WASP Antibody

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Protocols

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