Human/Mouse/Rat NADK Antibody

Catalog # Availability Size / Price Qty
AF8009
AF8009-SP
Detection of Human, Mouse, and Rat NADK by Western Blot.
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Human/Mouse/Rat NADK Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NADK
Met1-Gly446
Accession # O95544
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat NADK by Western Blot. View Larger

Detection of Human, Mouse, and Rat NADK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NADK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NADK at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NADK

NADK (Nicotinamide Adenine Dinucleotide Kinase; also Poly(P)/ATP NAD kinase) is a 49-52 kDa cytosolic member of the NAD kinase family of enzymes. Although it is widely expressed, it is not found in skeletal muscle. NADK forms homotetramers and catalyzes the generation of NADP from NAD through the hydrolysis of ATP. NADP plays a role in fatty acid synthesis plus steroidogenesis, and provides a substrate for the generation of NADPH that serves to regulate the expression of reductases and antioxidant proteins. Human NADK is 446 amino acids (aa) in length. It contains one kinase domain (aa 105-429) plus a C-terminal Ala- and Glu-rich segment. There are four utilized phosphorylation sites at Ser46, Ser48, Ser50 and Ser64. The potential exists for an alternate start site at Met134, and two isoform variants are reported, one that contain a 56 aa substitution for aa 1-88, and another that possesses a 105 aa substitution for Met88 coupled to a 41 aa insertion after Glu131. Full-length NADK (aa 1-446) shares 91% aa sequence identity with mouse NADK. Recently, a human mitochondrial NADK was identified (termed C5orf33), but found to share only 19% aa sequence identity with cytosolic NADK.

Long Name
NAD Kinase
Entrez Gene IDs
65220 (Human); 192185 (Mouse)
Alternate Names
dJ283E3.1; DKFZp686L22239; EC 2.7.1; EC 2.7.1.23; FLJ13052; FLJ37724; FLJ54695; FLJ77769; FLJ78247; FLJ78307; MGC1900; NAD kinase; NADK; Poly(P)/ATP NAD kinase

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