Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 866916
View all NEDD8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NEDD8
Leu2-Gly77
Accession # Q15843

Specificity

Detects human NEDD8 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat NEDD8 Antibody

Detection of Human, Mouse, and Rat NEDD8 antibody by Western Blot.

Detection of Human, Mouse, and Rat NEDD8 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB49361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NEDD8 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NEDD8 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

NEDD8 in HeLa Human Cell Line.

NEDD8 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat NEDD8 Polyclonal Antibody (Catalog # MAB49361) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat NEDD8 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line

Reviewed Applications

Read 1 review rated 5 using MAB49361 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD8

NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6‑8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with the cullin family of proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human proNEDD8 is 81 amino acids (aa) in length and contains one ubiquitin-like domain (aa 1‑65), and a 4‑5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and rat.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

Alternate Names

Neddylin, Rub1

Entrez Gene IDs

4738 (Human); 18002 (Mouse); 25490 (Rat)

Gene Symbol

NEDD8

UniProt

Q15843 (Human)

Additional NEDD8 Products

Product Documents for Human/Mouse/Rat NEDD8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat NEDD8 Antibody

For research use only

Customer Reviews for Human/Mouse/Rat NEDD8 Antibody (1)

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  • Human/Mouse/Rat NEDD8 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 01/24/2022
    Human/Mouse/Rat NEDD8 Antibody MAB49361

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Protocols

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