Human/Mouse/Rat NEDD8 Antibody

Catalog # Availability Size / Price Qty
MAB49361-100
MAB49361-SP
Human/Mouse/Rat NEDD8 Antibody in Western Blot
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Human/Mouse/Rat NEDD8 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human NEDD8 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 866916
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NEDD8
Leu2-Gly77
Accession # Q15843
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat NEDD8 by Western Blot. View Larger

Detection of Human, Mouse, and Rat NEDD8 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB49361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NEDD8 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry NEDD8 in HeLa Human Cell Line. View Larger

NEDD8 in HeLa Human Cell Line. NEDD8 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat NEDD8 Polyclonal Antibody (Catalog # MAB49361) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NEDD8

NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6‑8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with the cullin family of proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human proNEDD8 is 81 amino acids (aa) in length and contains one ubiquitin-like domain (aa 1‑65), and a 4‑5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and rat.

Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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