Human/Mouse/Rat p130Cas Antibody

    
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human p130Cas in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ajuba is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 685718
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human p130Cas
    Met66-Val412
    Accession # P56945
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human, Mouse, and Rat p130Cas by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human p130Cas Monoclonal Antibody (Catalog # MAB5730) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p130Cas at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p130Cas
p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105-130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Pro‑rich region (aa 74-87), at least 10 phosphotyrosine (aa 128-410) and four phosphoserine (aa 134-639) sites, an SH3 binding motif (aa 635-643) and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22 aa substitution for aa 1-4, an alternate start site at Met30, a deletion of aa 2-211, and a four aa substitution for aa 1-4 with a deletion of aa 65-212. Over aa 66-412, human p130Cas shares 91% aa identity with mouse p130Cas.    
  • Long Name:
    Breast Cancer Anti-estrogen Resistance 1
  • Entrez Gene IDs:
    9564 (Human); 12927 (Mouse); 25414 (Rat)
  • Alternate Names:
    BCAR1; breast cancer estrogen resistance 1; Cas scaffolding protein family member 1; CAS1; CASFLJ45059; CASS1; CASS1CRKASbreast cancer estrogen resistance protein 1; CRKAS; Crk-associated substrate p130Cas; CRK-associated substrate; FLJ12176; p130Cas
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 148  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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