Human/Mouse/Rat p53 Biotinylated Antibody
Human/Mouse/Rat p53 Biotinylated Antibody Summary
Accession # P04637
Recombinant Mouse p53
Recombinant Rat p53
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.
Citations for Human/Mouse/Rat p53 Biotinylated Antibody
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Scintillation proximity assay for total p53 protein as an alternative to ELISA.
Authors: Oberlander S, Xie T, Chandrachud U
J. Immunol. Methods, 2010;360(1):173-7.
Sample Types: Cell Lysates
Applications: scintillation proximity assay
In vitro analysis of DNA-protein interactions by proximity ligation.
Authors: Gustafsdottir SM, Schlingemann J, Rada-Iglesias A, Schallmeiner E, Kamali-Moghaddam M, Wadelius C, Landegren U
Proc. Natl. Acad. Sci. U.S.A., 2007;104(9):3067-72.
Sample Types: Cell Lysates
Applications: Proximity Ligation Assay
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