Human/Mouse/Rat p53 Antibody

Catalog # Availability Size / Price Qty
AF1355
AF1355-SP

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Detection of Human p53 by Western Blot.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse/Rat p53 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p53.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunoprecipitation
1-2 µg/500 µg cell lysate
CEM human T-lymphoblastoid cell line, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
p53 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inp53 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human p53 antibody by Western Blot. View Larger

Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line and MCF-7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of p53-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of p53-regulated Genes by Chromatin Immunoprecipitation. MCF-7 human breast cancer cell line treated with 300 nM camptothecin overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. p53/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.

Immunocytochemistry p53 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human p53 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human p53 by Simple WesternTM. Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p53 at approximately 59 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human p53 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human p53 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockdown Validated Detection of Human p53 by Knockdown Validated View Larger

Detection of Human p53 by Knockdown Validated Overexpression of Survivin in glioma cells affects cell cycle and proliferation in a p53 dependent manner. a Western blot analysis showing cell lysates from U87-MG and U87-MGshp53 after transduction with Survivin and control vectors. The membranes were blotted with anti-p53 (53 kDa), anti-p53s15 (53 kDa), anti-p21waf/cip (21 kDa), anti-gamma H2AX (16 kDa), anti-cyclin D1 (37 kDa) and E (42 kDa singlets +50 kDa doublets) antibodies and the signals comparing control and Survivin cells were measured. The relative band density (fold increase) obtained from the densitometric analysis and normalized to the corresponding control is depicted. alpha -actin (42 kDa) was used as an internal loading control. b Representative BrdU-incorporation assays of U87-MG (left panel) and U87-MGshp53 cells (right panel) with overexpression of Survivin (bottom) compared to the empty vector-transduced controls (top). The overall amount of BrdU-positive fractions as well as the relative levels of BrdU+ and BrdU− cells in fractions with DNA contents of 2n, 4n, 8n and >8n are depicted. c Proliferation index of the U87-MG and U87-MGshp53 cells transduced with Survivin and empty vector controls. Depicted are mean values ± SD. **p < 0.01. d BrdU-incorporation in U87-MG and U87-MGshp53 cell fractions with DNA content >4n. Depicted are mean values ± SD. **p < 0.01. All data were collected 72 h after transduction of cells Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3932-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

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Citations for Human/Mouse/Rat p53 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNF?
    Authors: Oppezzo, A;Monney, L;Kilian, H;Slimani, L;Maczkowiak-Chartois, F;Rosselli, F;
    Cell & bioscience
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Role of p53 in Cisplatin-Induced Myotube Atrophy
    Authors: Matsumoto C, Sekine H, Zhang N et al.
    International Journal of Molecular Sciences
  3. Calorie restriction alters the mechanisms of radiation-induced mouse thymic lymphomagenesis
    Authors: T Nakayama, M Sunaoshi, Y Shang, M Takahashi, T Saito, BJ Blyth, Y Amasaki, K Daino, Y Shimada, A Tachibana, S Kakinuma
    PLoS ONE, 2023-01-20;18(1):e0280560.
    Species: Mouse
    Sample Types: Protein
    Applications: Simple Western
  4. Aberrant phosphorylation inactivates Numb in breast cancer causing expansion of the stem cell pool
    Authors: Maria Grazia Filippone, Stefano Freddi, Silvia Zecchini, Silvia Restelli, Ivan Nicola Colaluca, Giovanni Bertalot et al.
    Journal of Cell Biology
  5. Non-targeting control for MISSION shRNA library silences SNRPD3 leading to cell death or permanent growth arrest
    Authors: Czarnek M, Sarad K, Kara? A et al.
    Molecular Therapy - Nucleic Acids
  6. Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice
    Authors: EM Haberl, R Pohl, L Rein-Fisch, M Höring, S Krautbauer, G Liebisch, C Buechler
    Lipids in Health and Disease, 2021-10-10;20(1):135.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. MYC Promotes Bone Marrow Stem Cell Dysfunction in Fanconi Anemia
    Authors: A Rodríguez, K Zhang, A Färkkilä, J Filiatraul, C Yang, M Velázquez, E Furutani, DC Goldman, B García de, G Garza-Mayé, K McQueen, LA Sambel, B Molina, L Torres, M González, E Vadillo, R Pelayo, WH Fleming, M Grompe, A Shimamura, S Hautaniemi, J Greenberge, S Frías, K Parmar, AD D'Andrea
    Cell Stem Cell, 2020-09-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Pseudopterosin and O-Methyltylophorinidine Suppress Cell Growth in a 3D Spheroid Co-Culture Model of Pancreatic Ductal Adenocarcinoma
    Authors: Bailu Xie, Jan Hänsel, Vanessa Mundorf, Janina Betz, Irene Reimche, Mert Erkan et al.
    Bioengineering (Basel)
  9. Germline mutation of MDM4, a major p53 regulator, in a familial syndrome of defective telomere maintenance
    Authors: E Toufektcha, V Lejour, R Durand, N Giri, I Draskovic, B Bardot, P Laplante, S Jaber, BP Alter, JA Londono-Va, SA Savage, F Toledo
    Sci Adv, 2020-04-10;6(15):eaay3511.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  10. Stress-Induced, p53-Mediated Tumor Growth Inhibition of Melanoma by Modulated Electrohyperthermia in Mouse Models without Major Immunogenic Effects
    Authors: Balázs Besztercei, Tamás Vancsik, Anett Benedek, Enikő Major, Mbuotidem J. Thomas, Csaba A. Schvarcz et al.
    International Journal of Molecular Sciences
  11. Modulated electro‐hyperthermia induced p53 driven apoptosis and cell cycle arrest additively support doxorubicin chemotherapy of colorectal cancer in vitro
    Authors: Tamas Vancsik, Gertrud Forika, Andrea Balogh, Eva Kiss, Tibor Krenacs
    Cancer Medicine
  12. Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells
    Authors: Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang
    International Journal of Oncology
  13. Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway
    Authors: ZP You, MJ Yu, YL Zhang, K Shi
    Mol Med Rep, 2018-11-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Oxidative stress induces p38MAPK-dependent senescence in the feto-maternal interface cells
    Authors: Jin Jin, Lauren Richardson, Samantha Sheller-Miller, Nanbert Zhong, Ramkumar Menon
    Placenta
  15. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
    Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
    Oncol Lett, 2018-04-13;15(6):9009-9016.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  16. Reciprocity of Action of Increasing Oct4 and Repressing p53 in Transdifferentiation of Mouse Embryonic Fibroblasts into Cardiac Myocytes
    Authors: Hongran Wang, Shuying Zhao, Michelle Barton, Todd Rosengart, Austin J. Cooney
    Cellular Reprogramming
  17. Chromosomal instability induced by increased BIRC5/Survivin levels affects tumorigenicity of glioma cells
    Authors: M Conde, S Michen, R Wiedemuth, B Klink, E Schröck, G Schackert, A Temme
    BMC Cancer, 2017-12-28;17(1):889.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. FAS apoptotic inhibitory molecule 2 is a stress-induced intrinsic neuroprotective factor in the retina
    Authors: Mercy Pawar, Boris Busov, Aaruran Chandrasekhar, Jingyu Yao, David N Zacks, Cagri G Besirli
    Cell Death & Differentiation
  19. Dysfunction of the MDM2/p53 axis is linked to premature aging
    Authors: D Lessel, D Wu, C Trujillo, T Ramezani, I Lessel, MK Alwasiyah, B Saha, FM Hisama, K Rading, I Goebel, P Schütz, G Speit, J Högel, H Thiele, G Nürnberg, P Nürnberg, M Hammerschm, Y Zhu, DR Tong, C Katz, GM Martin, J Oshima, C Prives, C Kubisch
    J. Clin. Invest., 2017-08-28;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Combining anti-miR-155 with chemotherapy for the treatment of lung cancers
    Authors: Katrien Van Roosbr
    Clin. Cancer Res, 2016-11-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Recombinant human neuregulin-1 beta is protective against radiation-induced myocardial cell injury
    Authors: QIANG ZHOU, WENBING HU, XINXIONG FEI, XUQUN HUANG, XI CHEN, DEQING ZHAO et al.
    Molecular Medicine Reports
  22. Growth hormone is permissive for neoplastic colon growth
    Proc Natl Acad Sci USA, 2016-05-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53.
    Authors: Wiedemuth R, Klink B, Topfer K, Schrock E, Schackert G, Tatsuka M, Temme A
    Mol Cancer, 2014-05-09;13(0):107.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism
    Authors: Lucia Napione, Marina Strasly, Claudia Meda, Stefania Mitola, Maria Alvaro, Gabriella Doronzo et al.
    Angiogenesis
  25. Optimization of Stability, Encapsulation, Release, and Cross-Priming of Tumor Antigen-Containing PLGA Nanoparticles
    Authors: Shashi Prasad, Virginia Cody, Jennifer K. Saucier-Sawyer, Tarek R. Fadel, Richard L. Edelson, Martin A. Birchall et al.
    Pharmaceutical Research
  26. Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell-based anti-tumor immunotherapy
    Authors: Shashi Prasad, Virginia Cody, Jennifer K. Saucier-Sawyer, W. Mark Saltzman, Clarence T. Sasaki, Richard L. Edelson et al.
    Nanomedicine: Nanotechnology, Biology and Medicine
  27. Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha.
    Authors: Tsoporis JN, Izhar S, Parker TG
    J. Biol. Chem., 2008-08-27;283(44):30174-83.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon.
    Authors: Ishida T, Sakaguchi I
    Biol. Pharm. Bull., 2007-05-01;30(5):928-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. TIMP-1 gene deficiency increases tumour cell sensitivity to chemotherapy-induced apoptosis.
    Authors: Davidsen ML, Wurtz SØ, Romer MU, Sorensen NM, Johansen SK, Christensen IJ, Larsen JK, Offenberg H, Brunner N, Lademann U
    Br. J. Cancer, 2006-10-23;95(8):1114-20.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat p53 Antibody

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Human/Mouse/Rat p53 Antibody
By Christian Ostalecki on 11/12/2021
Application: IHC Sample Tested: Melanoma tissue Species: Human

Human/Mouse/Rat p53 Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Human/Mouse/Rat p53 Antibody
By Punitha Senthil on 01/12/2018
Application: WB Sample Tested: PANC-1 human pancreatic carcinoma cell line Species: Human