p62/SQSTM1 antibodies are ideal for immunocytochemistry colocalization studies in autophagosomes. The unconjugated antibody detects human p62/SQSTM1 in direct ELISAs, and human, mouse and rat p62/SQSTM1 in Western Blots
Monoclonal Mouse IgG1 Clone # 864807
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human p62/SQSTM1 Asp368-Leu440 Accession # Q13501
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
p62/SQSTM1 in RAW 264.7 Mouse Cell Line. p62/SQSTM1 was detected in formaldehyde fixed RAW 264.7 mouse monocyte/macrophage cell line treated with LPS using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8028G) at 1:10 dilution overnight at 4 °C and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
SQSTM1 (Sequestrome-1), also known as p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. The 440 amino acid (aa) human SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. It contains numerous regulatory phosphorylation sites and a dimerization site. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget’s disease of bone, Parkinson’s and Alzheimer’s diseases, and cancers. Within aa 344-440, which includes the ubiquitin-binding domain, human SQSTM1 shares 100% aa sequence identity with mouse and rat SQSTM1.
Entrez Gene IDs:
8878 (Human); 18412 (Mouse); 113894 (Rat)
A170; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; ORCA; OSIL; oxidative stress induced like; p60PDB3; p62; p62B; Paget disease of bone 3; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome 1; sequestosome-1; SQSTM1; Ubiquitin-binding protein p62; ZIP3
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