Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC8028G
p62/SQSTM1 in RAW 264.7 Mouse Cell Line.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
p62/SQSTM1 antibodies are ideal for immunocytochemistry colocalization studies in autophagosomes. The unconjugated antibody detects human p62/SQSTM1 in direct ELISAs, and human, mouse and rat p62/SQSTM1 in Western Blots
Source
Monoclonal Mouse IgG1 Clone # 864807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p62/SQSTM1
Asp368-Leu440
Accession # Q13501
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
1:10 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry p62/SQSTM1 in RAW 264.7 Mouse Cell Line. View Larger

p62/SQSTM1 in RAW 264.7 Mouse Cell Line. p62/SQSTM1 was detected in formaldehyde fixed RAW 264.7 mouse monocyte/macrophage cell line treated with LPS using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8028G) at 1:10 dilution overnight at 4 °C and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: p62/SQSTM1

SQSTM1 (Sequestrome-1), also known as p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. The 440 amino acid (aa) human SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. It contains numerous regulatory phosphorylation sites and a dimerization site. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget’s disease of bone, Parkinson’s and Alzheimer’s diseases, and cancers. Within aa 344-440, which includes the ubiquitin-binding domain, human SQSTM1 shares 100% aa sequence identity with mouse and rat SQSTM1.

Long Name
Sequestosome 1
Entrez Gene IDs
8878 (Human); 18412 (Mouse); 113894 (Rat)
Alternate Names
A170; Autophagy Receptor P62; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; ORCA; OSIL; oxidative stress induced like; p60PDB3; p62; p62B; Paget disease of bone 3; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome 1; sequestosome-1; SQSTM1; Ubiquitin-binding protein p62; ZIP3

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of Fatty Acid Oxidation Promotes Macrophage Control of Mycobacterium tuberculosis
    Authors: P Chandra, L He, M Zimmerman, G Yang, S Köster, M Ouimet, H Wang, KJ Moore, V Dartois, JD Schilling, JA Philips
    MBio, 2020;11(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review