Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody Summary
Accession # Q13501
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
p62/SQSTM1 in RAW 264.7 Mouse Cell Line. p62/SQSTM1 was detected in formaldehyde fixed RAW 264.7 mouse monocyte/macrophage cell line treated with LPS using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8028G) at 1:10 dilution overnight at 4 °C and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
SQSTM1 (Sequestrome-1), also known as p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. The 440 amino acid (aa) human SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. It contains numerous regulatory phosphorylation sites and a dimerization site. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget’s disease of bone, Parkinson’s and Alzheimer’s diseases, and cancers. Within aa 344-440, which includes the ubiquitin-binding domain, human SQSTM1 shares 100% aa sequence identity with mouse and rat SQSTM1.
Product Specific Notices
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Citation for Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Inhibition of Fatty Acid Oxidation Promotes Macrophage Control of Mycobacterium tuberculosis
Authors: P Chandra, L He, M Zimmerman, G Yang, S Köster, M Ouimet, H Wang, KJ Moore, V Dartois, JD Schilling, JA Philips
Sample Types: Whole Cells
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