Human/Mouse/Rat p70 S6 Kinase Alexa Fluor® 488-conjugated Antibody Summary
Accession # M60725
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat p70 S6 Kinase Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8962G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: p70 S6 Kinase
p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20‑fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well asT389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1) (1-3). Over amino acids (aa) 1-502, human and mouse share 99% aa sequence identity.
- Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
- Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
- Fenton, T.R. and I.T. Gout (2011) Int. J. Biochem. Cell Biol. 43:47.
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