Human/Mouse/Rat PARL Antibody

Catalog # Availability Size / Price Qty
AF7306
AF7306-SP
Detection of Human and Mouse PARL by Western Blot.
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Human/Mouse/Rat PARL Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PARL in Western blots and detects recombinant human PARL in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PARL
Gly71-Arg167
Accession # Q9H300
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse PARL by Western Blot. View Larger

Detection of Human and Mouse PARL by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, MCF‑7 human breast cancer cell line, and mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PARL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7306) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PARL at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PARL

PARL (Presenilins-associated Rhomboid-like protein; also P beta ) is a 41-43 kDa member of the peptidase S54 family (alternatively, the 1 + 6 PARL subfamily; Rhomboid family) of molecules. It is ubiquitously expressed, being found in both embryo and adult. Rhomboid proteins are an ancient family of intramembrane-cleaving molecules that evolved as regulators of mitochondrial membrane dynamics. PARL is an inner mitochondrial-membrane embedded protein that appears to have at least two functions. First, it interacts with Opa1 and regulates apoptosis. Second, it appears to communicate to the nucleus information related to levels of mitochondrial stress. Human PARL is 379 amino acids (aa) in length. It consists of a mitochondrial targeting sequence (aa 1-52) plus a mature polypeptide (aa 53-379) that contains seven transmembrane segments and a 25 aa C-terminus that projects into the intermitochondrial membrane space. PARL undergoes two cleavages. The first occurs between Gly52Phe53 and removes the targeting sequence, generating a membrane-bound 37 kDa mature protein. The second occurs between Ser77Ala78, generating a membrane-bound 34 kDa processed form, plus a matrix-soluble 25 aa peptide (P beta -peptide; aa 53-77) that translocates to the nucleus. There is one potential splice variant that poseesses a Ser substitution for aa 203-253. Over aa 71-167, human PARL shares 92% aa sequence identity with mouse PARL.

Long Name
Presenilins Associated Rhomboid-like Protein
Entrez Gene IDs
55486 (Human); 381038 (Mouse); 287979 (Rat)
Alternate Names
EC 3.4.21.105; mitochondrial; PARL; presenilin associated, rhomboid-like; PRO2207; PSARL1; RHBDS1; rhomboid 7 homolog 1

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