Human/Mouse/Rat Paxillin Antibody

Catalog # Availability Size / Price Qty
AF4259
AF4259-SP
Detection of Human/Mouse/Rat Paxillin by Western Blot.
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Product Details
Citations (7)
FAQs
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Human/Mouse/Rat Paxillin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Paxillin in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Paxillin
Asn59-Ser274
Accession # P49023
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Paxillin antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Paxillin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Immunocytochemistry Paxillin antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Paxillin in HUVEC Human Cells. Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips and cytosol. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Paxillin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Paxillin by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Paxillin at approximately 70 & 69 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Paxillin

The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased Paxillin protein levels have also been reported in several malignancies, including renal, lung, and breast carcinomas.

Entrez Gene IDs
5829 (Human); 19303 (Mouse); 360820 (Rat)
Alternate Names
FLJ16691; Paxillin; PXN

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Citations for Human/Mouse/Rat Paxillin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells
    Authors: Chen Y, Tristan CA, Lu C et al.
    Nat Methods
  2. Cas phosphorylation regulates focal adhesion assembly
    Authors: Saurav Kumar, Amanda Stainer, Julien Dubrulle, Christopher Simpkins, Jonathan A Cooper
    eLife
  3. A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics
    Authors: Elizabeth M Steenkiste, Jason D Berndt, Carissa Pilling, Christopher Simpkins, Jonathan A Cooper
    eLife
  4. Platelet-derived growth factor receptor-alpha and Ras-related C3 botulinum toxin substrate-1 regulate mechano-responsiveness of lung fibroblasts
    Authors: Stephen E. McGowan, Diann M. McCoy
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  5. CAF-like state in primary skin fibroblasts with constitutional BRCA1 epimutation sheds new light on tumor suppressor deficiency-related changes in healthy tissue.
    Authors: Etzold A, Galetzka D, Weis E, Bartsch O, Haaf T, Spix C, Itzel T, Schweiger S, Strand D, Strand S, Zechner U
    Epigenetics, 2016-03-07;11(2):120-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Mechanoresponsive, omni-directional and local matrix-degrading actin protrusions in human mesenchymal stem cells microencapsulated in a 3D collagen matrix.
    Authors: Ho F, Zhang W, Li Y, Chan B
    Biomaterials, 2015-03-19;53(0):392-405.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis
    Authors: Antoniades I, Kyriakou M, Charalambous A, et al.
    Cell communication and signaling : CCS

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