Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PKA RI beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKA RI beta
Met1-Val381
Accession # P31321

Specificity

Detects human, mouse, and rat PKA RI beta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA RI alpha or rhPKA RII beta is observed; cross-reactivity with PKA RII alpha is unknown.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PKA RI beta Antibody

Detection of Human PKA RI beta antibody by Western Blot.

Detection of Human PKA RI beta by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PKA RI beta antibody by Western Blot.

Detection of Human PKA RI beta by Western Blot.

Western blot shows recombinant human PKA RIa, PKA RI beta, and PKA RII beta (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/ Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PKA RI beta antibody by Simple WesternTM.

Detection of Human PKA RI beta by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKA RI beta at approximately 56 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of PKA RI beta by Western Blot

Detection of PKA RI beta by Western Blot

ARHGAP36 downregulates PKAC levels.(a) MDCK cells transfected with YFP-ARHGAP36, or a YFP control, were fixed after 8 or 24 h and subjected to immunofluorescence using antibodies against GFP and PKAC. Images were collected by confocal microscopy. Scale bars, 10 μm. (b) HEK293T cells were transfected with YFP-ARHGAP36 or YFP-Cherry control for 24 h. Lysates were immunoblotted with the indicated antibodies. (c) HEK293T cells were transfected with YFP-ARHGAP36 or YFP control. Lysates were immunoblotted with antibodies against RI alpha, RI beta RII alpha, RII beta, GAPDH and GFP. (d) MDCK cells transfected with CFP-PKI were fixed after 24 h and subjected to immunofluorescence as in a. Scale bar, 10 μm. (e) MDCK cells transfected with YFP-delta N (195–516), YFP-N (1–194), YFP-N2 (118–195), YFP-RRV or Cherry-36i were fixed after 24 h and subjected to immunofluorescence as in a. 36i signal was not amplified using antibodies. Scale bars, 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27713425), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat PKA RI beta Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF4177 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKA RI beta

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, alpha  and beta. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name

cAMP-dependent Protein Kinase Regulatory Type I beta

Alternate Names

PRKAR1B, RIbeta

Entrez Gene IDs

5575 (Human); 19085 (Mouse); 25521 (Rat)

Gene Symbol

PRKAR1B

UniProt

P31321

Additional PKA RI beta Products

Product Documents for Human/Mouse/Rat PKA RI beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PKA RI beta Antibody

For research use only

Citations for Human/Mouse/Rat PKA RI beta Antibody

Customer Reviews for Human/Mouse/Rat PKA RI beta Antibody (1)

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  • Human/Mouse/Rat PKA RI beta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 11/04/2019
    Human/Mouse/Rat PKA RI beta Antibody AF4177

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