Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all POR/Cytochrome P450 Reductase Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived human POR/Cytochrome P450 Reductase
Arg45-Ser677
Accession # P16435

Specificity

Detects recombinant human POR/Cytochrome P450 Reductase in direct ELISAs and human, mouse, and rat POR/Cytochrome P450 Reductase in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody

Detection of Human, Mouse, and Rat POR/ Cytochrome P450 Reductase antibody by Western Blot.

Detection of Human, Mouse, and Rat POR/ Cytochrome P450 Reductase by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat POR/Cytochrome P450 Reductase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: POR/Cytochrome P450 Reductase

NADPH-Cytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and Antley-Bixler syndrome.

References

  1. Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
  2. Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
  3. Flueck C.E. et al. (2004) Nat. Genet. 36:228.

Long Name

NADPH Cytochrome P450 Reductase

Alternate Names

CPR, CYPOR, Cytochrome P450 Reductase, P450R

Entrez Gene IDs

5447 (Human)

Gene Symbol

POR

UniProt

P16435

Additional POR/Cytochrome P450 Reductase Products

Product Documents for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody

For research use only

Related Research Areas

Redox Enzymes

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