Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects recombinant human POR/Cytochrome P450 Reductase in direct ELISAs and human, mouse, and rat POR/Cytochrome P450 Reductase in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived human POR/Cytochrome P450 Reductase
    Arg45-Ser677
    Accession # P16435
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat POR/
Cytochrome P450 Reductase by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat POR/Cytochrome P450 Reductase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POR/Cytochrome P450 Reductase

NADPH-Cytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and Antley-Bixler syndrome.

  • References:
    1. Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
    2. Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
    3. Flueck C.E. et al. (2004) Nat. Genet. 36:228.
  • Long Name:
    NADPH Cytochrome P450 Reductase
  • Entrez Gene IDs:
    5447 (Human)
  • Alternate Names:
    CPR; CYPOR; CYPORFLJ26468; Cytochrome P450 Reductase; DKFZp686G04235; EC 1.6.2.4; NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; P450R; POR
Related Research Areas
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