Human/Mouse/Rat PP2C beta /PPM1B Antibody Summary
Accession # O75688
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat PP2C beta /PPM1B by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2C beta /PPM1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PP2C beta/PPM1B at approximately 53 and 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C beta/PPM1B
Protein phosphatase 2C beta (PP2C beta ), also called protein phosphatase, magnesium-dependent type 1B (PPM1B), preferentially dephosphorylates serine and threonine residues on proteins. There are five splice variants with sizes of 53, 43, 42, 36, and 21 kDa. This enzyme is resistant to inhibitors of other protein phosphatases such as okadaic acid and calyculin. Association of PP2C beta with I kappa B kinase inhibits its phosphorylating activity and is regulated by TNFalpha stimulation. PP2C beta is found in many cell types and tissues, especially muscle. The mRNA for PP2C beta accumulates in mature spermatocytes and metaphase-arrested, unfertilized oocytes. Knockout of PP2C beta in mice is lethal prior to embryo implantation.
Citation for Human/Mouse/Rat PP2C beta /PPM1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway.
Authors: Bruce D, Macartney T, Yong W, Shou W, Sapkota G
Cell Signal, 2012;24(11):1999-2006.
Sample Types: Cell Lysates
Applications: Western Blot
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