PPP2R4 Antibody (529245)

R&D Systems | Catalog # MAB5929

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 529245
View all PPP2R4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PPP2R4
Glu14-Asn323
Accession # Q15257

Specificity

Detects endogenous human, mouse and rat PPP2R4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PPP2R4 Antibody (529245)

Detection of Human/Mouse/Rat PPP2R4 antibody by Western Blot.

Detection of Human/Mouse/Rat PPP2R4 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PPP2R4 Monoclonal Antibody (Catalog # MAB5929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PPP2R4 at approximately 39 to 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PPP2R4 Antibody (529245)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PPP2R4

PPP2R4, also known as serine/threonine protein phosphatase 2A regulatory subunit B’, PR53 isoform, and PTPA, is a widely expressed member of the PTPA-type peptidyl prolyl isomerase family. Human PPP2R4 is 358 amino acids (aa) in length. Three splicing variants produce four isoforms for PPP2R4, and isoform 2 is the standard protein. Isoform 1, isoform 3 and isoform 4 have deletions corresponding to aa 73-107, 45-108, and 73-149 in isoform 2, respectively. Human PPP2R4 is 87% aa identical to mouse and rabbit PPP2R4. PPP2R4 reversibly stimulates the variable phosphotyrosyl phosphatase activity of the PP2A core heterodimer in the presence of ATP and Mg2+. It has been shown that p53 downregulates the expression of PPP2R4.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Alternate Names

PTPA

Entrez Gene IDs

5524 (Human); 110854 (Mouse)

Gene Symbol

PTPA

UniProt

Q15257

Additional PPP2R4 Products

Product Documents for PPP2R4 Antibody (529245)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PPP2R4 Antibody (529245)

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

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