Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Protocadherin-17 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Protocadherin-17
Leu18-Ser705
Accession # O14917

Specificity

Detects human, mouse and rat Protocadherin-17 in  Western blots. In direct ELISAs, less than 5% cross-reactivity with Protocadherin-10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Protocadherin-17 Antibody

Detection of Mouse and Rat Protocadherin-17 antibody by Western Blot.

Detection of Mouse and Rat Protocadherin-17 by Western Blot.

Western blot shows lysates of mouse brain (cortex) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Protocadherin-17 at approximately 120-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Protocadherin-17 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse brain (cortex) tissue and rat brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Protocadherin-17

Protocadherin 17 (PCDH-17) is a 120-150 kDa glycoprotein, delta 2-group member of the of the protocadherin subfamily, cadherin family of molecules. It is expressed on endothelium and epithelium such as stratified squamous epithelium and (likely) gastric columnar epithelium. PCDH17 is posited to act as an adhesion molecule, and appears to play a role in promoting cell cycle arrest and inhibiting migration. Mature human PCDH-17 is a type I transmembrane protein that is 1142 amino acids (aa) in length. It contains a 690 aa extracellular domain (ECD) (aa 18-707) plus a 431 aa cytoplasmic region. There are 6 cadherin domains in the ECD (aa 18-695). Two splice forms are reported that involve substitutions in the cytoplasmic region. The first possesses a 14 aa substitution, while the second shows a 25 aa substitution, both for aa 876-1159. Over aa 18-705, human PCDH-17 shares 98% aa identity with mouse PCDH-17.

Alternate Names

PCDH17, PCDH68, PCH68, Protocadherin17

Entrez Gene IDs

27253 (Human); 219228 (Mouse); 306055 (Rat)

Gene Symbol

PCDH17

UniProt

O14917 (Human)

Additional Protocadherin-17 Products

Product Documents for Human/Mouse/Rat Protocadherin-17 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Protocadherin-17 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

Customer Reviews for Human/Mouse/Rat Protocadherin-17 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Protocadherin-17 Antibody and earn rewards!

Have you used Human/Mouse/Rat Protocadherin-17 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies