|Detection of Mouse and Rat Protocadherin-17 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Protocadherin-17 at approximately 120-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Protocadherin 17 (PCDH-17) is a 120-150 kDa glycoprotein, δ2-group member of the of the protocadherin subfamily, cadherin family of molecules. It is expressed on endothelium and epithelium such as stratified squamous epithelium and (likely) gastric columnar epithelium. PCDH17 is posited to act as an adhesion molecule, and appears to play a role in promoting cell cycle arrest and inhibiting migration. Mature human PCDH-17 is a type I transmembrane protein that is 1142 amino acids (aa) in length. It contains a 690 aa extracellular domain (ECD) (aa 18-707) plus a 431 aa cytoplasmic region. There are 6 cadherin domains in the ECD (aa 18-695). Two splice forms are reported that involve substitutions in the cytoplasmic region. The first possesses a 14 aa substitution, while the second shows a 25 aa substitution, both for aa 876-1159. Over aa 18-705, human PCDH-17 shares 98% aa identity with mouse PCDH-17.
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