Human/Mouse/Rat PSMA2 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat PSMA2 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PSMA2
    Ala2-Ala234
    Accession # P25787
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat PSMA2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PSMA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7639) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMA2

PSMA2 (Proteasome subunit alpha type 2; also proteasome component C3 and Macropain subunit C3) is an intracellular 25-27 kDa member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700 kDa 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha -subunits, while the two central rings are composed of seven catalytic beta -subunits. The complex degrades 80-90% of all intracellular proteins through both a ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta -subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2.

  • Long Name:
    Proteasome (Prosome, Macropain) Subunit, alpha Type, 2
  • Entrez Gene IDs:
    5683 (Human); 19166 (Mouse); 29669 (Rat)
  • Alternate Names:
    HC3; MU; PMSA2; PSC3; PSMA2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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