PSMA2 (Proteasome subunit alpha type 2; also proteasome component C3 and Macropain subunit C3) is an intracellular 25-27 kDa member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700 kDa 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha -subunits, while the two central rings are composed of seven catalytic beta -subunits. The complex degrades 80-90% of all intracellular proteins through both a ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta -subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala2-Ala234
Accession # P25787
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat PSMA2 Antibody
Detection of Human, Mouse, and Rat PSMA2 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PSMA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7639) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse/Rat PSMA2 Antibody
Western Blot
Sample: A172 human glioblastoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line
Reviewed Applications
Read 1 review rated 4 using AF7639 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PSMA2
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PSMA2 Products
Product Documents for Human/Mouse/Rat PSMA2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat PSMA2 Antibody
For research use only
Related Research Areas
Customer Reviews for Human/Mouse/Rat PSMA2 Antibody (1)
Have you used Human/Mouse/Rat PSMA2 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
-
Application: ImmunohistochemistrySample Tested: HUVEC human umbilical vein endothelial cellsSpecies: HumanVerified Customer | Posted 12/08/2018
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars