Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. The unconjugated antibody detects endogenous human, mouse and rat Ribosomal Protein S6/RPS6 in Western blots.
Monoclonal Mouse IgG2B Clone # 522731
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Ribosomal Protein S6/RPS6 Met1-Lys249 Accession # P62753
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Ribosomal Protein S6/RPS6 in HeLa Human Cell Line.
Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5436G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ribosomal Protein S6/RPS6
40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.
Entrez Gene IDs:
6194 (Human); 20104 (Mouse); 29304 (Rat)
Phosphoprotein NP33, 40S ribosomal protein S6; pS6; ribosomal protein S6; RPS6; S6
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.