Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor® 488-conjugated Antibody IC5436G: R&D Systems

Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. The unconjugated antibody detects endogenous human, mouse and rat Ribosomal Protein S6/RPS6 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 522731
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Ribosomal Protein S6/RPS6
    Met1-Lys249
    Accession # P62753
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1:10 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry

Ribosomal Protein S6/RPS6 in HeLa Human Cell Line. Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5436G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ribosomal Protein S6/RPS6
40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.
  • Entrez Gene IDs:
    6194 (Human); 20104 (Mouse); 29304 (Rat)
  • Alternate Names:
    Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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