Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 522731
View all Ribosomal Protein S6/RPS6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RPS6
Met1-Lys249
Accession # P62753

Specificity

The antibody detects endogenous human, mouse and rat RPS6 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

Detection of Human and Rat Ribosomal Protein S6 antibody by Western Blot.

Detection of Human and Rat Ribosomal Protein S6 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Ribosomal Protein S6/RPS6 antibody in MM55K Mouse Cell Line by Immunocytochemistry (ICC).

Ribosomal Protein S6/RPS6 in MM55K Mouse Cell Line.

Ribosomal Protein S6/RPS6 was detected in immersion fixed MM55K mouse kidney cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Monoclonal Antibody (Catalog # MAB5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MM55K mouse kidney cell line

Western Blot

0.25 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cells

Reviewed Applications

Read 2 reviews rated 4 using MAB5436 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Alternate Names

RPS6

Entrez Gene IDs

6194 (Human); 20104 (Mouse); 29304 (Rat)

Gene Symbol

RPS6

UniProt

P62753

Additional Ribosomal Protein S6/RPS6 Products

Product Documents for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

For research use only

Related Research Areas

Interferons
Translation Regulation

Citations for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

Customer Reviews for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody (2)

4 out of 5
2 Customer Ratings
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Customer Images

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  • Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 11/30/2022
    Mouse bone marrow mesenchymal stromal cells lysed with NP40 buffer for protein extraction. 10 ug of proteins were loaded in a 10% Acrylamide gel.
    Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody MAB5436
  • Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody
    Name: Francesca Pacifici
    Application: Western Blot
    Sample Tested: Skeletal muscle tissue
    Species: Mouse
    Verified Customer | Posted 11/20/2017
    Western Blot: S6/RPS6 [MAB5436] - Total protein from mouse skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-S6/RPS6 1:2000 in non-fat milk.
    Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody MAB5436

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Protocols

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