|Detection of Human and Rat Ribosomal Protein S6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.|
|Ribosomal Protein S6/RPS6 in MM55K Mouse Cell Line. Ribosomal Protein S6/RPS6 was detected in immersion fixed MM55K mouse kidney cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Monoclonal Antibody (Catalog # MAB5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.
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