Detection of Human and Rat Ribosomal Protein S6 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Ribosomal Protein S6/RPS6 in MM55K Mouse Cell Line.
Ribosomal Protein S6/RPS6 was detected in immersion fixed MM55K mouse kidney cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Monoclonal Antibody (Catalog # MAB5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ribosomal Protein S6/RPS6
40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.
Entrez Gene IDs:
6194 (Human); 20104 (Mouse); 29304 (Rat)
Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6
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Western Blot: Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody [MAB5436].
Skeletal muscle tissue
Other Experimental Details
Other Experimental Details
Western Blot: S6/RPS6 [MAB5436] - Total protein from mouse skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-S6/RPS6 1:2000 in non-fat milk.