Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Rat Ribosomal Protein S6 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody Summary

Species Reactivity
Human, Mouse, Rat
The antibody detects endogenous human, mouse and rat RPS6 in Western blots.
Monoclonal Mouse IgG2B Clone # 522731
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human RPS6
Accession # P62753
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.25 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat Ribosomal Protein S6 antibody by Western Blot. View Larger

Detection of Human and Rat Ribosomal Protein S6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Ribosomal Protein S6/RPS6 antibody in MM55K Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Ribosomal Protein S6/RPS6 in MM55K Mouse Cell Line. Ribosomal Protein S6/RPS6 was detected in immersion fixed MM55K mouse kidney cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Monoclonal Antibody (Catalog # MAB5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Entrez Gene IDs
6194 (Human); 20104 (Mouse); 29304 (Rat)
Alternate Names
Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6

Product Datasheets

You must select a language.


Citation for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells.
    Authors: Alexis Autour, Sunny C Y Jeng, Adam D Cawte, Amir Abdolahza, Angela Galli, Shanker S S Panchapak, David Rueda, Michael Ryckelync, Peter J Unrau
    Nature Communications, 2018;0(0):2041-1723.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody

Average Rating: 4 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody
By Anonymous on 11/30/2022
Application: WB Sample Tested: Bone marrow cells Species: Mouse

Mouse bone marrow mesenchymal stromal cells lysed with NP40 buffer for protein extraction. 10 ug of proteins were loaded in a 10% Acrylamide gel.

Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody
By Francesca Pacifici on 11/20/2017
Application: WB Sample Tested: Skeletal muscle tissue Species: Mouse

Western Blot: S6/RPS6 [MAB5436] - Total protein from mouse skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-S6/RPS6 1:2000 in non-fat milk.