Human Ribosomal Protein S6/RPS6 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. Detects human Ribosomal Protein S6/RPS6 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 522731
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Ribosomal Protein S6/RPS6
    Met1-Lys249
    Accession # P62753
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Ribosomal Protein S6/RPS6 in HeLa Human Cell Line. Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Ribosomal Protein S6/RPS6 Biotinylated Monoclonal Antibody (Catalog # BAM5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

  • Entrez Gene IDs:
    6194 (Human); 20104 (Mouse); 29304 (Rat)
  • Alternate Names:
    Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6
Related Research Areas
Isotype Controls
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Mouse IgG2B Biotinylated Isotype Control

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Streptavidin-Alkaline Phosphatase

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NorthernLights Streptavidin NL637

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NorthernLights Streptavidin NL493

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EvenCoat Streptavidin Coated Plates, 5 Pack

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