Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 522731
View all Ribosomal Protein S6/RPS6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Ribosomal Protein S6/RPS6
Met1-Lys249
Accession # P62753

Specificity

Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. Detects human Ribosomal Protein S6/RPS6 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody

Ribosomal Protein S6/RPS6 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Ribosomal Protein S6/RPS6 in HeLa Human Cell Line.

Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Ribosomal Protein S6/RPS6 Biotinylated Monoclonal Antibody (Catalog # BAM5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Alternate Names

RPS6

Entrez Gene IDs

6194 (Human); 20104 (Mouse); 29304 (Rat)

Gene Symbol

RPS6

UniProt

P62753

Additional Ribosomal Protein S6/RPS6 Products

Product Documents for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody

For research use only

Related Research Areas

Interferons
Translation Regulation

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