Human/Mouse/Rat Smad7 Antibody

(13 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Smad7.
  • Source
    Monoclonal Mouse IgG2B Clone # 293039
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Smad7
    Gly320-Ser398
    Accession # O15105
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human/Mouse/Rat Smad7 by Western Blot. Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunohistochemistry
Smad7 in Human Liver. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Smad7 by Simple WesternTM. Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Smad7

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.

  • Long Name:
    Mothers Against DPP Homolog 7
  • Entrez Gene IDs:
    4092 (Human)
  • Alternate Names:
    CRCS3; FLJ16482; hSMAD7; MAD homolog 7; MAD homolog 8; MAD, mothers against decapentaplegic homolog 7 (Drosophila); MADH7; MADH7mothers against decapentaplegic homolog 7; MADH8; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMAD 7; SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; Smad7
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
Sample Type
  1. The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration
    J Exp Clin Cancer Res, 2016;35(1):122.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. TGF-Beta Induces Endometriotic Progression via a Non-Canonical, KLF11 Mediated Mechanism
    Endocrinology, 2016;0(0):en20161194.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Downregulation of the TGF-? pseudoreceptor BAMBI in non-small cell lung cancer enhances TGF-? signaling and invasion
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5.
    Authors: Bardita C, Predescu D, Sha F, Patel M, Balaji G, Predescu S
    J Cell Sci, 2015;128(8):1528-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy.
    Authors: Mehuron T, Kumar A, Duarte L, Yamauchi J, Accorsi A, Girgenrath M
    Skelet Muscle, 2015;4(0):14.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-beta/SMAD signaling.
    Authors: Devine D, Rostas J, Metge B, Das S, Mulekar M, Tucker J, Grizzle W, Buchsbaum D, Shevde L, Samant R
    Oncogene, 2014;33(20):2620-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition.
    Authors: Parikh A, Lee C, Joseph P, Marchini S, Baccarini A, Kolev V, Romualdi C, Fruscio R, Shah H, Wang F, Mullokandov G, Fishman D, D'Incalci M, Rahaman J, Kalir T, Redline R, Brown B, Narla G, DiFeo A
    Nat Commun, 2014;5(0):2977.
    Species: Human
    Sample Type: tissue
    Application: IHC Paraffin-embedded
  8. Angiotensin II upregulates Kv1.5 expression through ROS-dependent transforming growth factor-beta1 and extracellular signal-regulated kinase 1/2 signalings in neonatal rat atrial myocytes.
    Authors: Lu G, Xu S, Peng L, Huang Z, Wang Y, Gao X
    Biochem Biophys Res Commun, 2014;454(3):410-416.
    Species: Rat
    Sample Type: Cell Lysate
    Application: WB
  9. Protons sensitize epithelial cells to mesenchymal transition.
    Authors: Wang M, Hada M, Saha J, Sridharan D, Pluth J, Cucinotta F
    PLoS ONE, 2012;7(7):e41249.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. The TGF-β/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced intestinal injury independently of a Smad signaling pathway.
    Authors: Hneino M, Francois A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F
    PLoS ONE, 2012;7(5):e35672.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation.
    Authors: Su RW, Lei W, Liu JL
    PLoS ONE, 2010;5(11):e15513.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  12. Nuclear function of Smad7 promotes myogenesis.
    Authors: Miyake T, Alli NS, McDermott JC
    Mol. Cell. Biol., 2010;30(3):722-35.
    Species: Mouse
    Sample Type: Cell Lysate
    Application: WB
  13. 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins.
    Authors: Seong HA, Jung H, Kim KT, Ha H
    J. Biol. Chem., 2007;282(16):12272-89.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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