Human/Mouse/Rat Smad7 Antibody

Catalog # Availability Size / Price Qty
MAB2029
MAB2029-SP
Smad7 in Human Liver Cancer Tissue.
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Product Details
Citations (21)
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Human/Mouse/Rat Smad7 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Smad7.
Source
Monoclonal Mouse IgG2B Clone # 293039
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Smad7
Gly320-Ser398
Accession # O15105
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Smad7 in Human Liver Cancer Tissue. View Larger

Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse/Rat Smad7 by Western Blot. View Larger

Detection of Human/Mouse/Rat Smad7 by Western Blot. Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Smad7 in Human Liver. View Larger

Smad7 in Human Liver. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Smad7 by Simple  WesternTM. View Larger

Detection of Human Smad7 by Simple WesternTM. Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad7

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.

Long Name
Mothers Against DPP Homolog 7
Entrez Gene IDs
4092 (Human)
Alternate Names
CRCS3; FLJ16482; hSMAD7; MAD homolog 7; MAD homolog 8; MAD, mothers against decapentaplegic homolog 7 (Drosophila); MADH7; MADH7mothers against decapentaplegic homolog 7; MADH8; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMAD 7; SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; Smad7

Product Datasheets

Citations for Human/Mouse/Rat Smad7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. High SMAD7 and p-SMAD2,3 expression is associated with Environmental Enteropathy in children
    Authors: S Syed, V Dinallo, NT Iqbal, L Di Iorio, D Di Fusco, S Guleria, BC Amadi, K Sadiq, C Moskaluk, SA Ali, P Kelly, G Monteleone
    PLoS Negl Trop Dis, 2018;12(2):e0006224.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Smooth muscle cells differentiated from human mesenchymal stem cells regulated by microRNA (miR)-503 and miR-222-5p are suitable for vascular tissue engineering
    Authors: W Gu, X Hong, A Le Bras, WN Nowak, S Issa Bhalo, J Deng, Y Xie, Y Hu, XZ Ruan, Q Xu
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Hepatocyte-specific Smad7 deletion accelerates DEN-induced HCC via activation of STAT3 signaling in mice
    Authors: T Feng, J Dzieran, X Yuan, A Dropmann, T Maass, A Teufel, S Marhenke, T Gaiser, F Rückert, I Kleiter, S Kanzler, MP Ebert, A Vogel, P Ten Dijke, S Dooley, NM Meindl-Bei
    Oncogenesis, 2017;6(1):e294.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Smad7 knockdown activates protein kinase RNA-associated eIF2? pathway leading to colon cancer cell death
    Authors: V De Simone, G Bevivino, S Sedda, R Izzo, F Laudisi, V Dinallo, E Franzè, A Colantoni, A Ortenzi, S Salvatori, P Rossi, GS Sica, MC Fantini, C Stolfi, G Monteleone
    Cell Death Dis, 2017;8(3):e2681.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-? signaling in and promote progression of hepatocellular carcinoma
    Authors: H Sun, Z Peng, H Tang, D Xie, Z Jia, L Zhong, S Zhao, Z Ma, Y Gao, L Zeng, R Luo, K Xie
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Downregulation of the TGF-? pseudoreceptor BAMBI in non-small cell lung cancer enhances TGF-? signaling and invasion
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  7. TGF-Beta Induces Endometriotic Progression via a Non-Canonical, KLF11 Mediated Mechanism
    Endocrinology, 2016;0(0):en20161194.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  8. The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration
    J Exp Clin Cancer Res, 2016;35(1):122.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  9. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis
    Nat Commun, 2016;7(0):13498.
    Applications: WB
  10. MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells
    Authors: Boris Hinz
    Nat Mater, 2016;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  11. MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis.
    Authors: Murugaiyan G, da Cunha A, Ajay A, Joller N, Garo L, Kumaradevan S, Yosef N, Vaidya V, Weiner H
    J Clin Invest, 2015;125(3):1069-80.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  12. Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy.
    Authors: Mehuron T, Kumar A, Duarte L, Yamauchi J, Accorsi A, Girgenrath M
    Skelet Muscle, 2015;4(0):14.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  13. Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5.
    Authors: Bardita C, Predescu D, Sha F, Patel M, Balaji G, Predescu S
    J Cell Sci, 2015;128(8):1528-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  14. microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition.
    Authors: Parikh A, Lee C, Joseph P, Marchini S, Baccarini A, Kolev V, Romualdi C, Fruscio R, Shah H, Wang F, Mullokandov G, Fishman D, D'Incalci M, Rahaman J, Kalir T, Redline R, Brown B, Narla G, DiFeo A
    Nat Commun, 2014;5(0):2977.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  15. Angiotensin II upregulates Kv1.5 expression through ROS-dependent transforming growth factor-beta1 and extracellular signal-regulated kinase 1/2 signalings in neonatal rat atrial myocytes.
    Authors: Lu G, Xu S, Peng L, Huang Z, Wang Y, Gao X
    Biochem Biophys Res Commun, 2014;454(3):410-416.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  16. Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-beta/SMAD signaling.
    Authors: Devine D, Rostas J, Metge B, Das S, Mulekar M, Tucker J, Grizzle W, Buchsbaum D, Shevde L, Samant R
    Oncogene, 2014;33(20):2620-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  17. Protons sensitize epithelial cells to mesenchymal transition.
    Authors: Wang M, Hada M, Saha J, Sridharan D, Pluth J, Cucinotta F
    PLoS ONE, 2012;7(7):e41249.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  18. The TGF-β/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced intestinal injury independently of a Smad signaling pathway.
    Authors: Hneino M, Francois A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F
    PLoS ONE, 2012;7(5):e35672.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  19. Nuclear function of Smad7 promotes myogenesis.
    Authors: Miyake T, Alli NS, McDermott JC
    Mol. Cell. Biol., 2010;30(3):722-35.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  20. The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation.
    Authors: Su RW, Lei W, Liu JL
    PLoS ONE, 2010;5(11):e15513.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  21. 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins.
    Authors: Seong HA, Jung H, Kim KT, Ha H
    J. Biol. Chem., 2007;282(16):12272-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Mouse IgG2B Antibody

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