Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

Catalog # Availability Size / Price Qty
AF7275
AF7275-SP
Detection of Human, Mouse, and Rat ST3  beta -Gal  alpha -2,3-Sialyltransferase 2/ST3GAL2 by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ST6GALNAC4 and rhST3GAL1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2
Pro52-Gln350
Accession # NP_008858
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat ST3  beta -Gal  alpha -2,3-Sialyltransferase 2/ST3GAL2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to the Gal beta 1-3GalNAc structure to form NeuAc alpha 2-3Gal beta 1-3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3). Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3). ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage-specific embryonic antigen-4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6). The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase-coupled glycosyltransferase assay (7). Over aa 52-350 human ST3GAL2 share 94% aa sequence identity with mouse and rat.

References
  1. Varki, A. (1999) Glycobiology 2:25.
  2. Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
  3. Giordanengo, V. et al. (1997) Eur. J. Biochem. 247:558.
  4. Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
  5. Saito, S. et al. (2003) J. Biol. Chem. 278:26474.
  6. Suila, H. et al. (2011) J. Mol. Cell Biol. 3:99.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name
ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2
Entrez Gene IDs
6483 (Human); 20444 (Mouse); 64442 (Rat)
Alternate Names
Alpha 2,3-ST 2; Beta-galactoside alpha-2,3-sialyltransferase 2; beta-galactoside alpha-2,3-sialytransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2; EC 2.4.99.4; EC 2.4.99.7; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; Gal-NAc6S; sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase); Sialyltransferase 4B; SIAT4B; SIAT4-B; ST3 beta-galactoside alpha-2,3-sialyltransferase 2; ST3GAL2; ST3GalA.2; ST3GalA.2ST3Gal II; ST3GALII

Product Datasheets

Citation for Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model
    Authors: TR Karhadkar, D Pilling, N Cox, RH Gomer
    Sci Rep, 2017;7(1):15069.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody and earn rewards!

Have you used Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review