Human/Mouse/Rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ST6GALNAC4 and rhST3GAL1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2
    Pro52-Gln350
    Accession # NP_008858
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to the Gal beta 1-3GalNAc structure to form NeuAc alpha 2-3Gal beta 1-3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3). Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3). ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage-specific embryonic antigen-4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6). The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase-coupled glycosyltransferase assay (7). Over aa 52-350 human ST3GAL2 share 94% aa sequence identity with mouse and rat.

  • References:
    1. Varki, A. (1999) Glycobiology 2:25.
    2. Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
    3. Giordanengo, V. et al. (1997) Eur. J. Biochem. 247:558.
    4. Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
    5. Saito, S. et al. (2003) J. Biol. Chem. 278:26474.
    6. Suila, H. et al. (2011) J. Mol. Cell Biol. 3:99.
    7. Wu, Z.L. et al. (2011) Glycobiology 21:727.
  • Long Name:
    ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2
  • Entrez Gene IDs:
    6483 (Human); 20444 (Mouse); 64442 (Rat)
  • Alternate Names:
    Alpha 2,3-ST 2; Beta-galactoside alpha-2,3-sialyltransferase 2; beta-galactoside alpha-2,3-sialytransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2; EC 2.4.99.4; EC 2.4.99.7; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; Gal-NAc6S; sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase); Sialyltransferase 4B; SIAT4B; SIAT4-B; ST3 beta-galactoside alpha-2,3-sialyltransferase 2; ST3GAL2; ST3GalA.2; ST3GalA.2ST3Gal II; ST3GALII
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