Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2
Pro52-Gln350
Accession # NP_008858

Specificity

Detects human, mouse and rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ST6GALNAC4 and rhST3GAL1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images

Detection of Human, Mouse, and Rat ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 antibody by Western Blot.

Detection of Human, Mouse, and Rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 by Western Blot.

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human heart tissue, mouse heart tissue, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to the Gal beta 1-3GalNAc structure to form NeuAc alpha 2-3Gal beta 1-3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3). Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3). ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage-specific embryonic antigen-4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6). The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase-coupled glycosyltransferase assay (7). Over aa 52-350 human ST3GAL2 share 94% aa sequence identity with mouse and rat.

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
  3. Giordanengo, V. et al. (1997) Eur. J. Biochem. 247:558.
  4. Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
  5. Saito, S. et al. (2003) J. Biol. Chem. 278:26474.
  6. Suila, H. et al. (2011) J. Mol. Cell Biol. 3:99.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2

Alternate Names

Alpha 2,3-ST 2, Sialyltransferase 4B, SIAT4B, ST3GalA.2

Entrez Gene IDs

6483 (Human); 20444 (Mouse); 64442 (Rat)

Gene Symbol

ST3GAL2

UniProt

NP_008858

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

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