Human/Mouse/Rat Teneurin-4 Antibody

Catalog # Availability Size / Price Qty
AF6320
AF6320-SP

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Detection of Human and Mouse Teneurin‑4 by Western Blot.
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Product Details
Citations (3)
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Human/Mouse/Rat Teneurin-4 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Teneurin-4 in Western blots and human Teneurin-4 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Teneurin-1 is observed, and less than 1% cross-reactivity with rhTeneurin-2 and rhTeneurin-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Teneurin-4
Lys61-Lys340
Accession # Q6N022
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human and Mouse Teneurin‑4 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and L‑929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Teneurin‑4 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot View Larger

Detection of Rat Teneurin‑4 by Western Blot. Western blot shows lysates of Rat cortical stem cell. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Teneurin‑4 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Teneurin-4

Teneurin-4 (also Ten-m4, Doc4 and tenascin-M4) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is widely expressed in the embryo, and in adult, participates in cell-to-cell adhesion, and may communicate ER stress levels. Human Teneurin-4 is a 2769 amino acid (aa) type II TM glycoprotein. It contains a 345 aa cytoplasmic region (aa 1-345), plus a 2403 aa extracellular domain (ECD) (aa 367‑2769). The ECD possesses eight sequential EGF-like domains (aa 561-831), five NHL repeats, each of which form a beta -propeller (aa 1216-1566), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2726-2766) termed TCAP-1 that shows bioactivity. Teneurin-4 is hypothesized to form heterodimers with other teneurins. Over aa 61-340, human Teneurin-4 shares 95% aa identity with mouse Teneurin-4.

Entrez Gene IDs
26011 (Human)
Alternate Names
Doc4; ODZ4; Tenascin-M4; Teneurin4; Teneurin-4; Ten-M4; TNM4

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Citations for Human/Mouse/Rat Teneurin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Aquilaria crassna Leaf Extract Ameliorates Glucose-Induced Neurotoxicity In Vitro and Improves Lifespan in Caenorhabditis elegans
    Authors: N Pattaracho, N Sornkaew, W Warayanon, P Rangsinth, C Sillapacha, W Vongthip, S Chuchawank, A Prasansukl, T Tencomnao
    Nutrients, 2022-09-05;14(17):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Role and Involvement of TENM4 and miR-708 in Breast Cancer Development and Therapy
    Authors: G Peppino, F Riccardo, M Arigoni, E Bolli, G Barutello, F Cavallo, E Quaglino
    Cells, 2022-01-05;11(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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