Detects human, mouse, and rat Teneurin-4 in Western blots and human Teneurin-4 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Teneurin-1 is observed, and less than 1% cross-reactivity with rhTeneurin-2 and rhTeneurin-3 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human Teneurin-4 Lys61-Lys340 Accession # Q6N022
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat Teneurin‑4 by Western Blot. Western blot shows lysates of rat embryonic cortical neurons. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin‑4 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Teneurin-4 (also Ten-m4, Doc4 and tenascin-M4) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is widely expressed in the embryo, and in adult, participates in cell-to-cell adhesion, and may communicate ER stress levels. Human Teneurin-4 is a 2769 amino acid (aa) type II TM glycoprotein. It contains a 345 aa cytoplasmic region (aa 1-345), plus a 2403 aa extracellular domain (ECD) (aa 367‑2769). The ECD possesses eight sequential EGF-like domains (aa 561-831), five NHL repeats, each of which form a beta -propeller (aa 1216-1566), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2726-2766) termed TCAP-1 that shows bioactivity. Teneurin-4 is hypothesized to form heterodimers with other teneurins. Over aa 61-340, human Teneurin-4 shares 95% aa identity with mouse Teneurin-4.
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