Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7).
Transglutaminase 2/TGM2 Antibody
R&D Systems | Catalog # AF4376
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Knockout Validated, Western Blot, Simple Western, Immunoprecipitation
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Loading...
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 2/TGM2
Ala2-Ala687 (Asn533Thr, Leu655Val)
Accession # P21980
Ala2-Ala687 (Asn533Thr, Leu655Val)
Accession # P21980
Specificity
Detects human Transglutaminase 2/TGM2 in direct ELISAs and human, mouse, and rat Transglutaminase 2/TGM2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TGM3, rhTGM4, and rhTGM7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Transglutaminase 2/TGM2 Antibody
Detection of Human, Mouse, and Rat Transglutaminase 2/TGM2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Transglutaminase 2/TGM2 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line loaded at 0.2 mg/mL. A specific band was detected for Transglutaminase 2/TGM2 at approximately 82 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Transglutaminase 2/TGM2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.
A549 WT and Transglutaminase 2/TGM2 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by incubation with a donkey anti-sheep Alexa-fluor 555 coupled secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 0.2 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot Shows Human Transglutaminase 2/TGM2 Specificity Using Knockout Cell Line.
Western blot shows A549 cell line and Transglutaminase 2/TGM2 knockout A549 cell line (KO). A549 WT and Transglutaminase 2/TGM2 KO were cultured in serum free media, and 40 μg of protein from concentrated culture media were processed for western blot. Nitrocellulose membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by HRP-conjugated donkey anti-sheep IgG Secondary Antibody. A specific band was detected for Transglutaminase 2/TGM2 at approximately 75 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Western Blot Shows Human Transglutaminase 2/TGM2 Specificity Using Knockout Cell Line.
Western blot shows lysates of A549 cell line and Transglutaminase 2/TGM2 knockout A549 cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by HRP-conjugated donkey anti-sheep IgG Secondary Antibody. A specific band was detected for Transglutaminase 2/TGM2 at approximately 75 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of Transglutaminase 2/TGM2 by Immunoprecipitation.
PMA-treated A549 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) pre-coupled to Dynabeads protein G. Immunoprecipitated Transglutaminase 2/TGM2 was detected with Rabbit Anti-Transglutaminase 2/TGM2 Antibody.The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).Applications for Transglutaminase 2/TGM2 Antibody
Application
Recommended Usage
Immunoprecipitation
2 µg/1 mg cell lysate
Sample: Cell lysate of A549 cell line
Sample: Cell lysate of A549 cell line
Knockout Validated
Transglutaminase 2/TGM2 is specifically detected in the parental A549 cell line, but is not detectable in knockout A549 cell line.
Simple Western
5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Transglutaminase 2/TGM2
References
- Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
- Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
- Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
- Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
- Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Alternate Names
TGC, TGM2, Transglutaminase 2, tTG
Gene Symbol
TGM2
UniProt
Additional Transglutaminase 2/TGM2 Products
Product Documents for Transglutaminase 2/TGM2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Transglutaminase 2/TGM2 Antibody
For research use only
Related Research Areas
Citations for Transglutaminase 2/TGM2 Antibody
Powered by Bioz
Customer Reviews for Transglutaminase 2/TGM2 Antibody
There are currently no reviews for this product. Be the first to review Transglutaminase 2/TGM2 Antibody and earn rewards!
Have you used Transglutaminase 2/TGM2 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...