Human/Mouse/Rat Transglutaminase 2/TGM2 Antibody Summary
Ala2-Ala687 (Asn533Thr, Leu655Val)
Accession # P21980
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Transglutaminase 2/TGM2 by Western Blot.
Detection of Human, Mouse, and Rat Transglutaminase 2/TGM2 by Western Blot.Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Transglutaminase 2/TGM2 by Simple WesternTM.
12-230 kDa separation system.
Detection of Human Transglutaminase 2/TGM2 by Simple WesternTM.Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line loaded at 0.2 mg/mL. A specific band was detected for Transglutaminase 2/TGM2 at approximately 82 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 2/TGM2
Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7).
- Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
- Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
- Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
- Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
- Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Citation for Human/Mouse/Rat Transglutaminase 2/TGM2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
Sample Types: Whole Tissue
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