Detection of Human, Mouse, and Rat UBE2N/Ubc13 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HL‑60 human acute promyelocytic leukemia cell line, Hepa 1‑6 mouse hepatoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat UBE2N/Ubc13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8197) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBE2N/Ubc13 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a 16 kDa member of the Ubiquitin-conjugating (E2) enzyme family. UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin. UBE2N/Ubc13 localizes to both the nucleus and cytoplasm, and forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants. The UBE2N (Ubc13)/Uev1A complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor kappa B (NF-kappa B) activation. In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response. Pathologically, UBE2N (Ubc13)/Uev1a complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells. Human UBE2N/Ubc13 shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively.
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