UCH-L1/PGP9.5 Antibody

R&D Systems | Catalog # AF6007

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCH-L1/PGP9.5
Gln2-Ala223
Accession # P09936

Specificity

Detects human, mouse, and rat UCH-L1/PGP9.5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for UCH-L1/PGP9.5 Antibody

Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 antibody by Western Blot.

Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/ Rat UCH-L1/PGP9.5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UCH-L1/PGP9.5 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human and Mouse UCH-L1 antibody by Simple WesternTM.

Detection of Human and Mouse UCH‑L1 by Simple WesternTM.

Simple Western lane view shows lysates of A172 human glioblastoma cell line and Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UCH-L1/ PGP9.5 at approximately 30 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat UCH-L1/PGP9.5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6007) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for UCH-L1/PGP9.5 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line

Western Blot

1 µg/mL
Sample: A172 human glioblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UCH-L1/PGP9.5

Deubiquitination is a critical regulatory process in the ubiquitin-proteasome pathway (1). Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine proteases that catalyze the hydrolysis of a peptide bond at the C-terminal glycine of ubiquitin. Members of the UCH family have been implicated in a number of human diseases, including neurodegenerative diseases and cancers (2). Mutations of the UCH-L1 gene and alterations of the protein activity have been found to be associated with several neurodegenerative disorders, including Parkinson’s, Huntington’s and Alzheimer’s diseases (3). It is also implicated in cancer tumorigenesis, including lung, breast, liver, kidney, colorectal and ovarian cancers (4‑8). UCH-L1 is thought to be a tumor suppressor and biomarker for hepatocellular carcinoma and other digestive tumors.

References

  1. Wing, S. (2003) Int. J. Biochem. Cell Biol. 35:590.
  2. Ventii, KH and Wilkinson, KD (2008) Biochem. J. 414:161.
  3. Gong, B. and Leznik, E. (2007) Drug News Perspect. 20:365.
  4. Kim, H. et al. (2009) Oncogene 28:117.
  5. Wang, W. et al. (2008) Int. J. Oncol. 33:1037.
  6. Yu, J. et al. (2008) Hepatology 48:508.
  7. Kagara, I. et al. (2008) J. Urol. 180:343.
  8. Okochi-Takada, E. et al. (2006) Int. J. Cancer 119:1338.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 22223 (Mouse)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for UCH-L1/PGP9.5 Antibody

For research use only

Citations for UCH-L1/PGP9.5 Antibody

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