Human/Mouse SCAMP3 Antibody

Catalog # Availability Size / Price Qty
AF5344
AF5344-SP
Detection of Human/Mouse SCAMP3 by Western Blot.
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Human/Mouse SCAMP3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse SCAMP3 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SCAMP3
Met1-Leu170
Accession # O14828
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse SCAMP3 by Western Blot. View Larger

Detection of Human/Mouse SCAMP3 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and K562 human chronic myelogenous leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse SCAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5344) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCAMP3 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCAMP3

SCAMP3 (Secretory carrier membrane protein 3) is a 38‑40 kDa member of the SCAMP family of proteins. It is expressed in heart and skeletal muscle, plus fat, and functions in post-Golgi recycling and EGFR internalization pathways. Human SCAMP3 is 347 amino acids (aa) in length. It contains a cytoplasmic N-terminus (aa 1‑170) that shows three NPF repeats (binding sites for EH domain proteins), a heptad repeat of aliphatic aa's, and a potential SH3-binding motif. This is followed by four consecutive transmembrane domains (aa 171‑297) plus a C-terminal cytoplasmic region (aa 298‑347). It appears that phosphorylation of SCAMP3 on Tyr41 is a requisite for its interaction with EGFR. There are three potential splice forms. One shows a deletion of aa 23‑48, while two others show a one and 23 aa substitution for aa 131‑144 and 131‑260, respectively. Over aa 1‑170, human and mouse SCAMP3 are 91% aa identical.

Long Name
Secretory Carrier Membrane Protein 3
Entrez Gene IDs
10067 (Human); 24045 (Mouse); 65169 (Rat)
Alternate Names
propin 1; PROPIN1; SCAMP3; secretory carrier membrane protein 3C1orf3; secretory carrier-associated membrane protein 3

Product Datasheets

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Human/Mouse SCAMP3 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cell Lysates Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: 1XTBS 0.05% Tween, 5% milk
Dilution: 1:1000