Human/Mouse SCAMP3 Antibody

(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects endogenous human and mouse SCAMP3 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SCAMP3
    Met1-Leu170
    Accession # O14828
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse SCAMP3 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and K562 human chronic myelogenous leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse SCAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5344) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCAMP3 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCAMP3

SCAMP3 (Secretory carrier membrane protein 3) is a 38‑40 kDa member of the SCAMP family of proteins. It is expressed in heart and skeletal muscle, plus fat, and functions in post-Golgi recycling and EGFR internalization pathways. Human SCAMP3 is 347 amino acids (aa) in length. It contains a cytoplasmic N-terminus (aa 1‑170) that shows three NPF repeats (binding sites for EH domain proteins), a heptad repeat of aliphatic aa's, and a potential SH3-binding motif. This is followed by four consecutive transmembrane domains (aa 171‑297) plus a C-terminal cytoplasmic region (aa 298‑347). It appears that phosphorylation of SCAMP3 on Tyr41 is a requisite for its interaction with EGFR. There are three potential splice forms. One shows a deletion of aa 23‑48, while two others show a one and 23 aa substitution for aa 131‑144 and 131‑260, respectively. Over aa 1‑170, human and mouse SCAMP3 are 91% aa identical.

  • Long Name:
    Secretory Carrier Membrane Protein 3
  • Entrez Gene IDs:
    10067 (Human); 24045 (Mouse); 65169 (Rat)
  • Alternate Names:
    propin 1; PROPIN1; SCAMP3; secretory carrier membrane protein 3C1orf3; secretory carrier-associated membrane protein 3
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 WB Human Anonymous 10/26/2015
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Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: 1XTBS 0.05% Tween, 5% milk
Dilution: 1:1000

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