Human/Mouse Semaphorin 3F Antibody

Catalog # Availability Size / Price Qty
AF3237
AF3237-SP

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Detection of Mouse Semaphorin 3F by Western Blot.
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Product Details
Citations (1)
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Human/Mouse Semaphorin 3F Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Semaphorin 3F in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Semaphorin 3B and rmSemaphorin 3C is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3F
Ala19-Pro775 (Arg583Ala, Arg586Ala)
Accession # O88632
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Semaphorin 3F antibody by Western Blot. View Larger

Detection of Mouse Semaphorin 3F by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Semaphorin 3F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3237) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 3F at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3F

Semaphorin 3F (Sema 3F; previously Sema IV) is one of six Class 3 (secreted) semaphorins which in the mouse share 40‑50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema 3F is expressed in the developing nervous system, especially in the dorsal spinal cord (2, 3). In adults, Sema 3F is expressed in the lung and most other tissues (2). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade beta -propeller similar to that found in integrin molecules. Fourteen conserved cysteine residues and one or more N-glycosylation sites are thought to be critical for forming the secondary structure (4). Isoform A is missing aa 153‑183 within the Sema domain relative to the long form (isoform B) but appears to have similar activity. C-terminal to the Sema domain, Sema 3F has a basic domain, a cysteine‑knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and another basic domain at the C‑terminus. Dimerization and cleavage at the C-terminus are required for repulsing activity of class 3 semaphorins (5). Mouse Sema 3F shares 96%, 99%, 92%, 97% and 82% aa identity with human, rat, bovine, canine and chick Sema 3F, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema 3F signaling is transduced by type-A plexins, especially Plexin-A3, via interaction with neuropilin-2 (3, 6). Genetic disruption of either Sema 3F or neuropilin-2 alters motor axon trajectory to the ventral forelimb (3). Sema 3F is deleted or downregulated in many metastatic tumors. Restoration of Sema 3F decreases tumorigenicity, vascularization and adhesiveness, most likely through repulsive interactions, VEGF antagonism and downstream integrin regulation (7).

References
  1. Kruger, R.P. et al. (2005) Nature Rev. Mol. Cell Biol. 6:789.
  2. Eckhardt, F. and A. Meyerhans (1998) Neuroreport 9:3975.
  3. Huber, A.B et al. (2005) Neuron 48:949.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Adams, R.H. et al. (1997) EMBO J. 16:6077.
  6. Yaron, A. et al. (2005) Neuron 45:513.
  7. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
Entrez Gene IDs
6405 (Human); 20350 (Mouse); 315996 (Rat)
Alternate Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema III/F; sema IV; Sema3F; SEMA4; SEMA-IV; SEMAK; Semaphorin 3F; semaphorin III/F; semaphorin IV

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Citation for Human/Mouse Semaphorin 3F Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Genetically modified macrophages accelerate myelin repair
    Authors: MS Aigrot, C Barthelemy, S Moyon, G Dufayet-Ch, L Izagirre-U, B Gillet-Leg, S Tada, L Bayón-Cord, JC Chara, C Matute, N Cartier, C Lubetzki, V Tepav?evi?
    Embo Molecular Medicine, 2022-07-13;0(0):e14759.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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