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Human/Mouse SOX1 Antibody

Catalog #: MAB3369
2 Citations Datasheet / COA / SDS
Newer Version Available: AF3369
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Discontinued Product

MAB3369 has been discontinued and is replaced by AF3369.

Detection of Human/Mouse SOX1 by Western Blot.
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Citations (2)
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Human/Mouse SOX1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse SOX1 in Western blots. In Western blots, this antibody shows approximately 10% cross-reactivity with rhSOX-2, and no cross-reactivity with rhSOX-3, -5, -6, -7, -9, -10, -11, -15, -17, or rmSOX-21.
Source
Monoclonal Mouse IgG1 Clone # 340303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX1
Asn242-Gly379 (Leu276Ile)
Accession # O00570
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse SOX1 antibody by Western Blot. View Larger

Detection of Human/Mouse SOX1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOX1 Monoclonal Antibody (Catalog # MAB3369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOX1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX1

SOX1 (SRY-related HMG box 1) is a 39 kDa transcription factor that belongs to the SRY family of HMG box proteins of which there are 30 members in vertebrates. Group B contains SOX1 along with SOX2, SOX3, SOX14 and SOX21; each of these factors share greater than 90% homology in their respective HMG box region. SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

Long Name
SRY-related HMG-box 1
Entrez Gene IDs
6656 (Human); 20664 (Mouse)
Alternate Names
SOX1; SRY (sex determining region Y)-box 1; SRY-related HMG-box gene 1; transcription factor SOX-1

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Citations for Human/Mouse SOX1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A CD44(high)/EGFR(low) Subpopulation within Head and Neck Cancer Cell Lines Shows an Epithelial-Mesenchymal Transition Phenotype and Resistance to Treatment.
    Authors: La Fleur L, Johansson A, Roberg K
    PLoS ONE, 2012-09-25;7(9):e44071.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism.
    Authors: Cho A, Ko HW, Eggenschwiler JT
    Dev. Biol., 2008-06-11;321(1):27-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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