Human/Mouse SOX1 Antibody

MAB3369 has been discontinued and is replaced by AF3369.
(2 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects endogenous human and mouse SOX1 in Western blots. In Western blots, this antibody shows approximately 10% cross-reactivity with rhSOX-2, and no cross-reactivity with rhSOX-3, -5, -6, -7, -9, -10, -11, -15, -17, or rmSOX-21.
  • Source
    Monoclonal Mouse IgG1 Clone # 340303
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOX1
    Asn242-Gly379 (Leu276Ile)
    Accession # O00570
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse SOX1 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOX1 Monoclonal Antibody (Catalog # MAB3369) followed by HRP-conjugated Anti‑Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOX1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX1

SOX1 (SRY-related HMG box 1) is a 39 kDa transcription factor that belongs to the SRY family of HMG box proteins of which there are 30 members in vertebrates. Group B contains SOX1 along with SOX2, SOX3, SOX14 and SOX21; each of these factors share greater than 90% homology in their respective HMG box region. SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

  • Long Name:
    SRY-related HMG-box 1
  • Entrez Gene IDs:
    6656 (Human); 20664 (Mouse)
  • Alternate Names:
    SOX1; SRY (sex determining region Y)-box 1; SRY-related HMG-box gene 1; transcription factor SOX-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. A CD44(high)/EGFR(low) Subpopulation within Head and Neck Cancer Cell Lines Shows an Epithelial-Mesenchymal Transition Phenotype and Resistance to Treatment.
    Authors: La Fleur L, Johansson A, Roberg K
    PLoS ONE, 2012;7(9):e44071.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism.
    Authors: Cho A, Ko HW, Eggenschwiler JT
    Dev. Biol., 2008;321(1):27-39.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh

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    MAB3369 has been replaced by
    AF3369

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