Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Canine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rabbit, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human SOX1
Asn242-Gly379 (Leu276Ile)
Accession # NP_005977

Specificity

Detects human SOX1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat SOX1 Antibody

Detection of Human SOX1 antibody by Western Blot.

Detection of Human SOX1 by Western Blot.

Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SOX1 antibody in ectoderm differentuiated BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

SOX1 in ectoderm differentuiated BG01V Human Embryonic Stem Cells.

SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

SOX1 antibody in differentiated NTera-2 Human Cell Line by Immunocytochemistry (ICC).

SOX1 in differentiated NTera‑2 Human Cell Line.

SOX1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

SOX1 in Rat Cortical Stem Cells.

SOX1 was detected in immersion fixed rat cortical stem cells (NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 antibody in Mouse Cortical Stem Cells by Immunocytochemistry (ICC).

SOX1 in Mouse Cortical Stem Cells.

SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 in Human Brain (Cortex).

SOX1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neuron. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human SOX1 antibody by Simple WesternTM.

Detection of Human SOX1 by Simple WesternTM.

Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at approximately 50 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, DQuantification of percentage of SOX1+ (C) and SOX2+ (D) cells in culture. The efficiency of neural induction is more than 99% in FKRP‐ and corrected‐iPSC (5D17, 5D23, and 3B17) lines. Data are mean ± s.d. n = 4 technical replicates.E, FFKRP‐ and corrected‐NSC lines can be further differentiated to cortical neural progenitor cells, expressing PAX6, OTX2, and vimentin.G–IQuantification of percentage of PAX6+ (G) and OTX2+ (H) cells in culture. About 91‐98% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express PAX6 (G). About 93‐96% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express OTX2 (H). Of the OTX2+ population, about 60‐67% cells are also Ki67+ cycling progenitors (I). Data are mean ± s.d. n = 4 technical replicates.J, KGlutamatergic projection neurons derived from FKRP and corrected (5D17, 5D23, and 3B17) progenitor cells. The vast majority of neurons contain vGlut1+ punctae in their neurites (labeled by Tuj1). Right panels are enlarged images from the insets of left panels.Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, DQuantification of percentage of SOX1+ (C) and SOX2+ (D) cells in culture. The efficiency of neural induction is more than 99% in FKRP‐ and corrected‐iPSC (5D17, 5D23, and 3B17) lines. Data are mean ± s.d. n = 4 technical replicates.E, FFKRP‐ and corrected‐NSC lines can be further differentiated to cortical neural progenitor cells, expressing PAX6, OTX2, and vimentin.G–IQuantification of percentage of PAX6+ (G) and OTX2+ (H) cells in culture. About 91‐98% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express PAX6 (G). About 93‐96% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express OTX2 (H). Of the OTX2+ population, about 60‐67% cells are also Ki67+ cycling progenitors (I). Data are mean ± s.d. n = 4 technical replicates.J, KGlutamatergic projection neurons derived from FKRP and corrected (5D17, 5D23, and 3B17) progenitor cells. The vast majority of neurons contain vGlut1+ punctae in their neurites (labeled by Tuj1). Right panels are enlarged images from the insets of left panels.Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). Scale bar, 1 mm.(B) Prosencephalon labeled for NSUN2 and SOX1. Region in squares are magnified in (b′) and (b″). Arrows indicate NSUN2-positive cells. Scale bar, 100 μm.(C–F) Bright-field image (C) and immunofluorescence (D–F) of AF22 (upper panels) and Sai1 (lower panels) cells labeled for Nestin (D), SOX2 (E), and beta III-tubulin (F). Scale bar, 50 μm.(G and H) NES cells co-labeled for NSUN2 and Nestin (NES) (G) or SOX1 (H).(I) Differentiation protocol.(J–L) Differentiated AF22 and Sai1 cells (day 15) labeled for Nestin (NES; J), SOX2 (K), and beta III-tubulin (L). Scale bars: 50 μm.(M) Western blot for NSUN2, beta III-tubulin (TUBB3), GFAP, SOX2, and Nestin during differentiation (days). alpha -Tubulin served as loading control.Nuclei are counterstained with DAPI (A, B, D–F, J–L). Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213671116302764), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). Scale bar, 1 mm.(B) Prosencephalon labeled for NSUN2 and SOX1. Region in squares are magnified in (b′) and (b″). Arrows indicate NSUN2-positive cells. Scale bar, 100 μm.(C–F) Bright-field image (C) and immunofluorescence (D–F) of AF22 (upper panels) and Sai1 (lower panels) cells labeled for Nestin (D), SOX2 (E), and beta III-tubulin (F). Scale bar, 50 μm.(G and H) NES cells co-labeled for NSUN2 and Nestin (NES) (G) or SOX1 (H).(I) Differentiation protocol.(J–L) Differentiated AF22 and Sai1 cells (day 15) labeled for Nestin (NES; J), SOX2 (K), and beta III-tubulin (L). Scale bars: 50 μm.(M) Western blot for NSUN2, beta III-tubulin (TUBB3), GFAP, SOX2, and Nestin during differentiation (days). alpha -Tubulin served as loading control.Nuclei are counterstained with DAPI (A, B, D–F, J–L). Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213671116302764), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/Immunofluorescence

GPI anchored proteins are required for neural differentiation.(A). Representative example of images of hiPSC-derived EBs and EB-derived rosettes during neural differentiation. Neural induction and rosette formation upon neural induction was assessed in three cell lines using a serum-free EB generation method. On day 2 (left) of hiPSC-derived EBs from PIGAwt, PIGAc.1234C>T, and PIGAnull after forced aggregation (20X magnification, scale bar is 50μm). On day 4 (middle), single homogeneous hiPSC-EBs collected were pooled in a 10 cm plate (4X magnification, scale bar is 100μm). On day 11 (right), neuroepithelial cells appeared and neural tube-like rosettes formed (EB-derived rosettes) and scale bar is 50μm. (B). Neural induction rates from EB-derived rosettes. The percentage of EB derived rosettes was 88.8% ± 4.6, 75.5% ± 9.8 and 68.4% ± 6.9 for PIGAwt, PIGAc.1234C>T, and PIGAnull, respectively. PIGAwt versus PIGAc.1234C>T (p>0.05, NS) and PIGAwt versus PIGAnull (*p<0.05, one way ANOVA and Multiple comparisons). Neural induction from PIGAnull hiPSCs was less than 70%. All values were mean ±SD. (C). Representative confocal images showing expression of neuron stem cell marker SOX1 (in red) combined proliferation by EdU labeling in hNPCs derived from isolated neural rosettes. Nuclei were visualized with DAPI (blue) and scale bar 100μm. (D). Representative confocal images showing expression of neuron progenitor marker PAX6 (in red) and combined proliferation by EdU (in green) in hNPCs derived from isolated neural rosettes. Nuclei were visualized with DAPI (blue) and scale bar 200μm. hNPCs from PIGAnull cell lines showed reduced expression of SOX1 and PAX6. (E). Proliferation rate in hNPCs was assessed and plotted in all three cell lines. EdU positive cells were counted and normalized by total number of nuclei staining with DAPI (blue). Proliferation was significantly decreased in PIGAnull and PIGAc.1234C>T compared to PIGAwt. (F). Graphs depict the percentage of positive cells for SOX-1 (left) and Pax6 (right) in hNPCs derived from PIGAwt, PIGAc.1234C>T, and PIGAnull hiPSC lines. The hNPCs derived from the PIGAnull hiPSCs showed significantly decreased expression of SOX1 and PAX6. Similar levels of SOX1 and PAX6 were expressed in hNPCs from PIGAwt and PIGAc.1234C>T. All values represent mean ± SD. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOX1 by Western Blot

Detection of SOX1 by Western Blot

Loss of DNMT3B impairs meso-endoderm lineage commitment. A Schematic representation of the two-step differentiation model from ESCs to EpiLCs first with Fgf beta & Activin A, & then to meso-endoderm (ME) progenitors with iGsk3. The time points of ’ collection are reported in the colour-code used throughout the figures (i.e., shades of grey for WT, shades of orange for 3BKO). b Hierarchical clustering of RNA-seq data from the in vitro differentiation & in vivo embryonic tissues derived from pre- & post-implantation mouse embryos35. Pearson’s correlation distance & Ward’s method employed to perform the analysis. c On the left, an RNA-seq heatmap showing the results of gene expression profiles clustering with K-means for WT & 3BKO (two independent clones) during the complete differentiation time course (ESC-EpiLC-ME). DEGs arising during the differentiation time course in any group identified by ANOVA-like test with edgeR54. Rows are genes, columns are samples & the scaled expression level (Z-score RPKM) is plotted. On the right, heatmap showing selected GO terms for enriched biological processes in each cluster. Terms related to meso-endoderm are highlighted in red. d Gene expression time-course for stage-specific pluripotency (naive, primed) & germ layers (mesoderm, endoderm, ectoderm) marker genes. Dots represent normalized RPKM values, averaged by replicates/conditions (n = 2 biological replicates per genotype or clone at each time point). Error bars represent standard errors. e, WB analysis of the de novo DNMTs (Dnmt3a1, Dnmt3a2, Dnmt3b), T (mesodermal marker) & Sox1 (neuro-ectodermal marker) expression during the differentiation time course. beta -actin serves as loading control. Representative of two independent experiments. Uncropped gels are provided in Supplementary Fig. 11. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36690616), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX1 by Immunocytochemistry/ Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/ Immunofluorescence

Characterization of NS/PCs derived from hiPSCs. b, Representative images (b) of immunocytochemical analysis of hiPSC-NS/PCs (NS/PC-A & NS/PC-B) using antibodies against SOX1, SOX2, & NESTIN. Inset: Hoechst nuclear staining of the same field. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37286713), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOX1 by Western Blot

Detection of SOX1 by Western Blot

Loss of DNMT3B impairs meso-endoderm lineage commitment. A Schematic representation of the two-step differentiation model from ESCs to EpiLCs first with Fgf beta & Activin A, & then to meso-endoderm (ME) progenitors with iGsk3. The time points of ’ collection are reported in the colour-code used throughout the figures (i.e., shades of grey for WT, shades of orange for 3BKO). b Hierarchical clustering of RNA-seq data from the in vitro differentiation & in vivo embryonic tissues derived from pre- & post-implantation mouse embryos35. Pearson’s correlation distance & Ward’s method employed to perform the analysis. c On the left, an RNA-seq heatmap showing the results of gene expression profiles clustering with K-means for WT & 3BKO (two independent clones) during the complete differentiation time course (ESC-EpiLC-ME). DEGs arising during the differentiation time course in any group identified by ANOVA-like test with edgeR54. Rows are genes, columns are samples & the scaled expression level (Z-score RPKM) is plotted. On the right, heatmap showing selected GO terms for enriched biological processes in each cluster. Terms related to meso-endoderm are highlighted in red. d Gene expression time-course for stage-specific pluripotency (naive, primed) & germ layers (mesoderm, endoderm, ectoderm) marker genes. Dots represent normalized RPKM values, averaged by replicates/conditions (n = 2 biological replicates per genotype or clone at each time point). Error bars represent standard errors. e, WB analysis of the de novo DNMTs (Dnmt3a1, Dnmt3a2, Dnmt3b), T (mesodermal marker) & Sox1 (neuro-ectodermal marker) expression during the differentiation time course. beta -actin serves as loading control. Representative of two independent experiments. Uncropped gels are provided in Supplementary Fig. 11. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36690616), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX1 by Immunocytochemistry/ Immunofluorescence

Detection of Human SOX1 by Immunocytochemistry/ Immunofluorescence

Characterization of NS/PCs derived from hiPSCs. b, Representative images (b) of immunocytochemical analysis of hiPSC-NS/PCs (NS/PC-A & NS/PC-B) using antibodies against SOX1, SOX2, & NESTIN. Inset: Hoechst nuclear staining of the same field. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37286713), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat SOX1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line, mouse cortical stem cells, rat cortical stem cells, and BG01V human embryonic stem cells differentiated into neural progenitor cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Simple Western

10 µg/mL
Sample: iB6J human iPS cells differentiated into neuroprogenitor cells

Western Blot

1 µg/mL
Sample: iB6J human iPS cells differentiated into neuroprogenitor cells

Reviewed Applications

Read 4 reviews rated 5 using AF3369 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX1

SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

Long Name

SRY-related HMG-box 1

Alternate Names

SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1, transcription factor SOX-1

Entrez Gene IDs

6656 (Human); 20664 (Mouse)

Gene Symbol

SOX1

UniProt

Additional SOX1 Products

Product Documents for Human/Mouse/Rat SOX1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat SOX1 Antibody

For research use only

Citations for Human/Mouse/Rat SOX1 Antibody

Customer Reviews for Human/Mouse/Rat SOX1 Antibody (4)

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4 Customer Ratings
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Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat SOX1 Antibody
    Name: Ying Chen
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: neural stem cells derived from h9
    Species: Human
    Verified Customer | Posted 08/19/2019
    Neural stem cells derived from H9 cells were fixed in 4% PFA for 15 minutes. The cells were incubated in the sox1 antibody at the concentration of 5ug/ml overnight at 4 degree, the followed with secondary antibody incubation at room temperature for 1hour.
    Human/Mouse/Rat SOX1 Antibody AF3369
  • Human/Mouse/Rat SOX1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: hESC-derived NSC
    Species: Human
    Verified Customer | Posted 03/21/2018
    SOX1 was detected human embryonic stem cells-derived neural stem cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 1 µg/mL for overnight at 4°C. Cells were stained using the Donkey anti-Goat IgG (H+L) Cross-Adsorbed, Alexa Fluor® 568 and counterstained with DAPI (blue).
    Human/Mouse/Rat SOX1 Antibody AF3369
  • Human SOX1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human NSC
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 1% BSA + 0.3% Triton X-100 in PBS<br />Dilution: 1/100
    Human/Mouse/Rat SOX1 Antibody AF3369
  • Human SOX1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human fibroblast and human neural stem cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 1% BSA + 0.3% Triton X-100 in PBS<br />Dilution: 1/100
    Human/Mouse/Rat SOX1 Antibody AF3369

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Showing  1 - 4 of 4 reviews Showing All

Protocols

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