Human/Mouse/Rat SOX1 Antibody

  (23 citations)
(3 Reviews)
    
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Product Details
Citations (23)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human SOX1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) SOX2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SOX1
    Asn242-Gly379 (Leu276Ile)
    Accession # NP_005977
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SOX1 by Western Blot. Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
SOX1 in ectoderm differentuiated BG01V Human Embryonic Stem Cells. SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunocytochemistry
SOX1 in differentiated NTera‑2 Human Cell Line. SOX1 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX1 in Rat Cortical Stem Cells. SOX1 was detected in immersion fixed rat cortical stem cells (Catalog # NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX1 in Mouse Cortical Stem Cells. SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human SOX1 by Simple WesternTM. Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells andiBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at approximately 50 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX1
SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.
  • Long Name:
    SRY-related HMG-box 1
  • Entrez Gene IDs:
    6656 (Human); 20664 (Mouse)
  • Alternate Names:
    SOX1; SRY (sex determining region Y)-box 1; SRY-related HMG-box gene 1; transcription factor SOX-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018;22(4):543-558.e12.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells
    Authors: JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim
    Exp. Mol. Med., 2018;50(4):24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease
    Authors: L Marrone, C Bus, D Schöndorf, JC Fitzgerald, M Kübler, B Schmid, P Reinhardt, L Reinhardt, M Deleidi, T Levin, A Meixner, B Klink, M Glatza, CJ Gloeckner, T Gasser, J Sternecker
    PLoS ONE, 2018;13(3):e0192497.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells
    Authors: M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo
    Stem Cell Res Ther, 2018;9(1):67.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Dynamic behaviour of human neuroepithelial cells in the developing forebrain
    Authors: L Subramania, M Bershteyn, MF Paredes, AR Kriegstein
    Nat Commun, 2017;8(0):14167.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  6. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia
    Authors: M Bershteyn, TJ Nowakowski, AA Pollen, E Di Lullo, A Nene, A Wynshaw-Bo, AR Kriegstein
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  7. Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state
    Authors: Rui Gao
    Biomaterials, 2017;119(0):53-67.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC
  8. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
    PLoS ONE, 2017;12(4):e0174074.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes
    Authors: ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar
    Am. J. Hum. Genet., 2017;100(4):617-634.
    Application: ICC
  10. Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells
    Authors: Y Li, R Wang, N Qiao, G Peng, K Zhang, K Tang, JJ Han, N Jing
    J. Biol. Chem., 2017;292(48):19590-19604.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells
    Authors: P Walsh, V Truong, C Hill, ND Stoflet, J Baden, WC Low, SA Keirstead, JR Dutton, AM Parr
    Cell Transplant, 2017;26(12):1890-1902.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
    Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
    PLoS ONE, 2017;12(2):e0171947.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A
    Stem Cells Transl Med, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  14. Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and�Motility
    Stem Cell Reports, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Immunofluoresence
  15. Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
    Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S
    PLoS ONE, 2015;10(8):e0136350.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  16. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.
    Authors: Paull D, Sevilla A, Zhou H, Hahn A, Kim H, Napolitano C, Tsankov A, Shang L, Krumholz K, Jagadeesan P, Woodard C, Sun B, Vilboux T, Zimmer M, Forero E, Moroziewicz D, Martinez H, Malicdan M, Weiss K, Vensand L, Dusenberry C, Polus H, Sy K, Kahler D, Gahl W, Solomon S, Chang S, Meissner A, Eggan K, Noggle S
    Nat Methods, 2015;12(9):885-92.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  17. iPSC-Derived neural stem cells act via kinase inhibition to exert neuroprotective effects in spinal muscular atrophy with respiratory distress type 1.
    Authors: Simone C, Nizzardo M, Rizzo F, Ruggieri M, Riboldi G, Salani S, Bucchia M, Bresolin N, Comi G, Corti S
    Stem Cell Reports, 2014;3(2):297-311.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC No Specified
  18. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression.
    Authors: Ferrarese R, Harsh G, Yadav A, Bug E, Maticzka D, Reichardt W, Dombrowski S, Miller T, Masilamani A, Dai F, Kim H, Hadler M, Scholtens D, Yu I, Beck J, Srinivasasainagendra V, Costa F, Baxan N, Pfeifer D, von Elverfeldt D, Backofen R, Weyerbrock A, Duarte C, He X, Prinz M, Chandler J, Vogel H, Chakravarti A, Rich J, Carro M, Bredel M
    J Clin Invest, 2014;124(7):2861-76.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
    Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P
    Stem Cells Transl Med, 2014;3(12):1410-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  20. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  21. Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
    Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
    PLoS ONE, 2013;8(3):e59252.
  22. Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice.
    Authors: Vue TY, Bluske K, Alishahi A, Yang LL, Koyano-Nakagawa N, Novitch B, Nakagawa Y
    J. Neurosci., 2009;29(14):4484-97.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  23. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
    Authors: Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P
    Am J Hum Genet, 0;100(4):617-634.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 5 (Based on 3 reviews)

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Read what people are saying who have used Human/Mouse/Rat SOX1 Antibody.

We have 3 review tested in 1 species: Human.
We have 3 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Excellent
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ICC/IF
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Human
(3)
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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX1 Antibody AF3369 Array AF3369
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Excellent
 ICC/IF Human Anonymous 03/21/2018
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX1 Antibody AF3369
Immunocytochemistry/Immunofluorescence: Human/Mouse/Rat SOX1 Antibody [AF3369].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested hESC-derived NSC
Species Human

Other Experimental Details

Other Experimental Details SOX1 was detected human embryonic stem cells-derived neural stem cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 1 µg/mL for overnight at 4°C. Cells were stained using the Donkey anti-Goat IgG (H+L) Cross-Adsorbed, Alexa Fluor® 568 and counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence Human SOX1 Antibody AF3369 Array AF3369
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Excellent
 ICC/IF Human Anonymous 10/26/2015
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Image Details
Immunocytochemistry/Immunofluorescence Human SOX1 Antibody AF3369
Immunocytochemistry/Immunofluorescence: Human SOX1 Antibody [AF3369].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested human NSC
Species Human

Other Experimental Details

Other Experimental Details Specificity: Specific
Sensitivity: Sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100
Immunocytochemistry/Immunofluorescence Human SOX1 Antibody AF3369 Array AF3369
Enlarge
Excellent
 ICC/IF Human Anonymous 10/26/2015
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Image Details
Immunocytochemistry/Immunofluorescence Human SOX1 Antibody AF3369
Immunocytochemistry/Immunofluorescence: Human SOX1 Antibody [AF3369].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested human fibroblast and human neural stem cells
Species Human

Other Experimental Details

Other Experimental Details Specificity: Specific
Sensitivity: Sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100

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