Human/Mouse STAT4 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and murine STAT4. Does not cross-react with any of the other STAT proteins.
  • Source
    Polyclonal Rabbit Serum
  • Formulation
    Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide.
  • Label
  • Western Blot
    1:2000 dilution
    STAT4 immunoprecipitated from lysates of human peripheral blood lymphocytes
  • Immunoprecipitation
    3 µg/500 µg cell lysate
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation of Human STAT4. STAT4 was immunoprecipitated from lysates (1 x 106 - 107 cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

  • Long Name:
    Signal Transducer and Activator of Transcription 4
  • Entrez Gene IDs:
    6775 (Human); 20849 (Mouse)
  • Alternate Names:
    signal transducer and activator of transcription 4; SLEB11
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
    Authors: Liao W, Lin JX, Wang L, Li P, Leonard WJ
    Nat. Immunol., 2011;12(6):551-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

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