Human/Mouse STAT4 Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Immunoprecipitation of Human STAT4. STAT4 was immunoprecipitated from lysates (1 x 106- 107cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.
Citation for Human/Mouse STAT4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
Authors: Liao W, Lin JX, Wang L, Li P, Leonard WJ
Nat. Immunol., 2011;12(6):551-9.
Sample Types: Cell Lysates
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