Human/Mouse STAT4 Antibody

Catalog # Availability Size / Price Qty
PA-ST4
Human/Mouse STAT4 Antibody in Immunoprecipitation
1 Image
Product Details
Citations (1)
FAQs
Reviews

Human/Mouse STAT4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and murine STAT4. Does not cross-react with any of the other STAT proteins.
Source
Polyclonal Rabbit Serum
Formulation
Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide.

Applications

Recommended Concentration
Sample
Western Blot
1:2000 dilution
STAT4 immunoprecipitated from lysates of human peripheral blood lymphocytes
Immunoprecipitation
3 µg/500 µg cell lysate
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunoprecipitation Immunoprecipitation of Human STAT4. View Larger

Immunoprecipitation of Human STAT4. STAT4 was immunoprecipitated from lysates (1 x 106- 107cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name
Signal Transducer and Activator of Transcription 4
Entrez Gene IDs
6775 (Human); 20849 (Mouse)
Alternate Names
signal transducer and activator of transcription 4; SLEB11; STAT4

Product Datasheets

Citation for Human/Mouse STAT4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
    Authors: Liao W, Lin JX, Wang L, Li P, Leonard WJ
    Nat. Immunol., 2011;12(6):551-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP

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