Human/Mouse TOR PE‑conjugated Antibody

Name: Human/Mouse TOR PE‑conjugated Antibody
Brand: R&D Systems
SKU: IC1537P

R&D Systems | Catalog # IC1537P

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Product Documents

Discontinued Product

IC1537P has been discontinued. View all TOR products.

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG_2A Clone # 303728

View all TOR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345

Specificity

Detects human TOR in direct ELISAs. Detects human and mouse TOR in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human/Mouse TOR PE‑conjugated Antibody

Detection of TOR in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with Anti-CD3, Anti-CD28, Recombinant Human TGF-beta 1 (Catalog # 240-B), and Recombinant Human IL-2 (Catalog # 202-IL) were stained with Rat Anti-Human/Mouse TOR PE-conjugated Monoclonal Antibody (Catalog # IC1537P) and Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of TOR in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with Anti-CD3, Anti-CD28, Recombinant Human TGF-beta 1 (Catalog # 240-B), and Recombinant Mouse IL-2 (Catalog # 402-ML) were stained with Rat Anti-Human/Mouse TOR PE-conjugated Monoclonal Antibody (Catalog # IC1537P) and Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse TOR PE‑conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and Recombinant Human IL‑2 (Catalog # 202-IL) and Mouse splenocytes treated with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and Recombinant Mouse IL‑2 (Catalog # 402-ML) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TOR

TOR, also known as FRAP1, is a member of the PI 3-Kinase-related Kinase (PIKK) family. It is the protein target of Rapamycin, an anti-rejection drug used in transplantation and a promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-Kinase/Akt signal transduction pathway.