Human/Mouse TOR PE-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human TOR in direct ELISAs. Detects human and mouse TOR in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 303728
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TOR
    Phe1720-Ala2020
    Accession # P42345
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry

Detection of TOR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and Recombinant Human IL‑2 (Catalog # 202-IL) were stained with Rat Anti-Human/Mouse TOR PE‑conjugated Monoclonal Antibody (Catalog # IC1537P) and Mouse Anti-Human CD25/IL-2 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of TOR in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and Recombinant Mouse IL‑2 (Catalog # 402-ML) were stained with Rat Anti-Human/Mouse TOR PE‑conjugated Monoclonal Antibody (Catalog # IC1537P) and Rat Anti-Mouse CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB554C). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TOR
TOR, also known as FRAP1, is a member of the PI 3-Kinase-related Kinase (PIKK) family. It is the protein target of Rapamycin, an anti-rejection drug used in transplantation and a promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-Kinase/Akt signal transduction pathway.
  • Long Name:
    Mammalian Target of Rapamycin
  • Entrez Gene IDs:
    2475 (Human); 287871 (Rat)
  • Alternate Names:
    EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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