Human/Mouse VAMP-2 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB5136N Datasheet / COA / SDS

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FAB5136N-100UG

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Human/Mouse VAMP-2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse VAMP‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human VAMP-1, -5, -7, or -8 is observed.

Source

Monoclonal Mouse IgG2b Clone # 541405

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human VAMPâ€‘2
Ser2-Lys94
Accession # P63027

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: VAMP-2

VAMP-2 (vesicle associated membrane protein 2; also Synaptobrevin-2) is a 13 kDa member of the Synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to Synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Long Name

Vesicle-Associated Membrane Protein 2

Entrez Gene IDs

6844 (Human); 22318 (Mouse); 24803 (Rat)

Alternate Names

FLJ11460; SYB2; SYB2vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; VAMP2; VAMP-2; VAMP-2vesicle associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)