Human/Mouse VAMP-2 Antibody

Catalog # Availability Size / Price Qty
MAB5136
MAB5136-SP
Detection of Human and Mouse VAMP‑2 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse VAMP-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse VAMP‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human VAMP-1, -5, -7, or -8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 541405
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VAMP‑2
Ser2-Lys94
Accession # P63027
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human spinal cord

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse VAMP-2 antibody by Western Blot. View Larger

Detection of Human and Mouse VAMP‑2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and mouse brain tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse VAMP-2 Monoclonal Antibody (Catalog # MAB5136) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-2

VAMP-2 (vesicle associated membrane protein 2; also Synaptobrevin-2) is a 13 kDa member of the Synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to Synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Long Name
Vesicle-Associated Membrane Protein 2
Entrez Gene IDs
6844 (Human); 22318 (Mouse); 24803 (Rat)
Alternate Names
FLJ11460; SYB2; SYB2vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; VAMP2; VAMP-2; VAMP-2vesicle associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)

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Citations for Human/Mouse VAMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway
    Authors: LJ Davis, NA Bright, JR Edgar, MDJ Parkinson, L Wartosch, J Mantell, AA Peden, JP Luzio
    Journal of Cell Science, 2021;134(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation
    Authors: JR Knudsen, DE Steenberg, JR Hingst, LR Hodgson, C Henriquez-, Z Li, B Kiens, EA Richter, JFP Wojtaszews, P Verkade, TE Jensen
    Mol Metab, 2020;0(0):100998.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Ca(2+)-dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages
    Authors: N Vashi, SB Andrabi, S Ghanwat, M Suar, D Kumar
    J. Biol. Chem, 2017;292(13):5144-5165.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse VAMP-2 Antibody

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Human/Mouse VAMP-2 Antibody
By Anonymous on 12/20/2021
Application: WB Sample Tested: Brain tissue Species: Mouse