Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 541405
View all VAMP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VAMP‑2
Ser2-Lys94
Accession # P63027

Specificity

Detects human and mouse VAMP‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human VAMP-1, -5, -7, or -8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse VAMP‑2 Antibody

Detection of Human and Mouse VAMP-2 antibody by Western Blot.

Detection of Human and Mouse VAMP‑2 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and mouse brain tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse VAMP-2 Monoclonal Antibody (Catalog # MAB5136) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse VAMP‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord

Western Blot

0.1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and mouse brain tissue

Reviewed Applications

Read 1 review rated 5 using MAB5136 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAMP-2

VAMP-2 (vesicle associated membrane protein 2; also Synaptobrevin-2) is a 13 kDa member of the Synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to Synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Long Name

Vesicle-Associated Membrane Protein 2

Alternate Names

SYB2, VAMP2

Entrez Gene IDs

6844 (Human); 22318 (Mouse); 24803 (Rat)

Gene Symbol

VAMP2

UniProt

P63027

Additional VAMP-2 Products

Product Documents for Human/Mouse VAMP‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse VAMP‑2 Antibody

For research use only

Citations for Human/Mouse VAMP‑2 Antibody

Customer Reviews for Human/Mouse VAMP‑2 Antibody (1)

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  • Human/Mouse VAMP-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 12/20/2021
    Human/Mouse VAMP‑2 Antibody MAB5136

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Protocols

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FAQs

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