Wnt-10a Antibody (342811)

R&D Systems | Catalog # MAB34691

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 342811
View all Wnt-10a Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-10a
Leu55-Glu95 and Gln278-Arg360
Accession # P70701

Specificity

Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 or rhWnt-7a is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Wnt-10a Antibody (342811)

Detection of Human and Mouse Wnt-10a antibody by Western Blot.

Detection of Human and Mouse Wnt‑10a by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-10a Monoclonal Antibody (Catalog # MAB34691) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-10a at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Wnt-10a Antibody (342811)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-10a

Wnt-10a is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid (aa) precursor that contains a 382 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic epithelium.

Long Name

Wingless-type MMTV Integration Site Family Member 10A

Alternate Names

Wnt10a

Entrez Gene IDs

80326 (Human); 22409 (Mouse); 316527 (Rat)

Gene Symbol

WNT10A

UniProt

P70701

Additional Wnt-10a Products

Product Documents for Wnt-10a Antibody (342811)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Wnt-10a Antibody (342811)

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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