Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant
mouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 or
rhWnt-7a is observed.
Monoclonal Rat IgG1 Clone # 342811
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Wnt-10a Leu55-Glu95 and Gln278-Arg360 Accession # P70701
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Wnt‑10a by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt‑10a Monoclonal Antibody (Catalog # MAB34691) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt‑10a at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
is a presumably secreted, glycosylated member of the Wnt family of
developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid
(aa) precursor that contains a 382 aa mature region. The mature region
contains 24 cysteines and two potential N-linked glycosylation sites.
Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature
human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence
identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic
Wingless-type MMTV Integration Site Family Member 10A
Entrez Gene IDs:
80326 (Human); 22409 (Mouse); 316527 (Rat)
FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A; Wnt10a
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.