ZNF281 (zinc finger protein 281; also ZBP99 and GZP1) is a 100 kDa member of the Krueppel C2H2-type Zn-finger family of proteins. It is found in embryonic stem cells, placenta, and lymphocytes, and appears to be necessary for the maintenance of pluripotency in stem cells. ZNF281 both represses (STAT3) and activates (Nanog) multiple genes, and is known to form a complex with Nanog, Oct4 and Sox2. Human ZFN281 is 895 amino acids in length. It contains one Gly-rich region (aa 4‑37), a poly-Pro motif (aa 90‑96) and four consecutive C2H2-type zinc finger domains (aa 261‑367). There are phosphorylation sites at Ser255, 395, 484, 785 and 807. Potential alternate start sites exist at Met296 and Met434. Over aa 681‑895, human ZFN281 shares 99% aa identity with mouse ZFN281.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
aa 681-895
Accession # Q9Y2X9
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse ZNF281 Antibody
Detection of Human and Mouse ZNF281 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line. Gels were loaded with whole cell lysate (WCL), cytoplasmic (Cyto), and nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ZNF281 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZNF281 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse ZNF281 Antibody
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ZNF281
Long Name
Alternate Names
Gene Symbol
UniProt
Additional ZNF281 Products
Product Documents for Human/Mouse ZNF281 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse ZNF281 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars