Human/Mouse ZNF281 Antibody Summary
Accession # Q9Y2X9
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse ZNF281 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line. Gels were loaded with whole cell lysate (WCL), cytoplasmic (Cyto), and nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ZNF281 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZNF281 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ZNF281 (zinc finger protein 281; also ZBP99 and GZP1) is a 100 kDa member of the Krueppel C2H2-type Zn-finger family of proteins. It is found in embryonic stem cells, placenta, and lymphocytes, and appears to be necessary for the maintenance of pluripotency in stem cells. ZNF281 both represses (STAT3) and activates (Nanog) multiple genes, and is known to form a complex with Nanog, Oct4 and Sox2. Human ZFN281 is 895 amino acids in length. It contains one Gly-rich region (aa 4‑37), a poly-Pro motif (aa 90‑96) and four consecutive C2H2-type zinc finger domains (aa 261‑367). There are phosphorylation sites at Ser255, 395, 484, 785 and 807. Potential alternate start sites exist at Met296 and Met434. Over aa 681‑895, human ZFN281 shares 99% aa identity with mouse ZFN281.
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