Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ZNF281 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZNF281
aa 681-895
Accession # Q9Y2X9

Specificity

Detects human and mouse ZNF281 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse ZNF281 Antibody

Detection of Human and Mouse ZNF281 antibody by Western Blot.

Detection of Human and Mouse ZNF281 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line. Gels were loaded with whole cell lysate (WCL), cytoplasmic (Cyto), and nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ZNF281 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZNF281 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse ZNF281 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZNF281

ZNF281 (zinc finger protein 281; also ZBP99 and GZP1) is a 100 kDa member of the Krueppel C2H2-type Zn-finger family of proteins. It is found in embryonic stem cells, placenta, and lymphocytes, and appears to be necessary for the maintenance of pluripotency in stem cells. ZNF281 both represses (STAT3) and activates (Nanog) multiple genes, and is known to form a complex with Nanog, Oct4 and Sox2. Human ZFN281 is 895 amino acids in length. It contains one Gly-rich region (aa 4‑37), a poly-Pro motif (aa 90‑96) and four consecutive C2H2-type zinc finger domains (aa 261‑367). There are phosphorylation sites at Ser255, 395, 484, 785 and 807. Potential alternate start sites exist at Met296 and Met434. Over aa 681‑895, human ZFN281 shares 99% aa identity with mouse ZFN281.

Long Name

Zinc Finger Protein 281

Alternate Names

GZP1, ZBP-99, ZNP-99

Entrez Gene IDs

23528 (Human); 226442 (Mouse); 305083 (Rat)

Gene Symbol

ZNF281

UniProt

Q9Y2X9 (Human)

Additional ZNF281 Products

Product Documents for Human/Mouse ZNF281 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse ZNF281 Antibody

For research use only

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