Human MSH2 Antibody

MSH2 (MutS Homolog 2) is a 100-106 kDa member of the mutS family of DNA mismatch repair molecules. It is a DNA binding protein that is expressed in rapidly proliferating cells, and acts in concert with multiple subunits. MSH2 forms a heterodimer with MSH6, forming MutS alpha. This dimer recognizes and repairs both nucleotide misparings and one or two aberrant nucleotide insertions/deletions. MSH2 also heterodimerizes with MSH3, forming MutS beta. This complex acts on DNA double-stranded breaks, and repairs large nucleotide insertions/deletions (<15 bases). MutS alpha is associated with BLM:p53:RAD51 complexes, while MSH2 beta is associated with SLX4/BTBD12 complexes. Human MSH2 is 934 amino acids (aa) in length. It contains three MutS domains (aa 18-132; 158-284; 297-612) plus an ABC transporter signature motif that may hydrolyze ATP (aa 633-852). There are multiple splice variants. One shows a premature truncation after His429, while others contain a 46 aa substitution for aa 879-934, a 29 aa substitution for aa 462-934, a 28 aa substitution for aa 783-934, a 3 aa substitution for aa 532-934, a 5 aa substitution for aa 482‑934 and a 31 aa substitution for aa 379-934. Over aa 1-140, human MSH2 shares 94% aa identity with mouse MSH2.