Human MSH2 Antibody

Catalog # Availability Size / Price Qty
AF6780
AF6780-SP
Detection of Human MSH2 by Western Blot.
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Human MSH2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MSH2 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MSH2
Ala2-Asp140
Accession # P43246
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human MSH2 by Western Blot. View Larger

Detection of Human MSH2 by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and SW480 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human MSH2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6780) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MSH2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSH2

MSH2 (MutS Homolog 2) is a 100-106 kDa member of the mutS family of DNA mismatch repair molecules. It is a DNA binding protein that is expressed in rapidly proliferating cells, and acts in concert with multiple subunits. MSH2 forms a heterodimer with MSH6, forming MutS alpha. This dimer recognizes and repairs both nucleotide misparings and one or two aberrant nucleotide insertions/deletions. MSH2 also heterodimerizes with MSH3, forming MutS beta. This complex acts on DNA double-stranded breaks, and repairs large nucleotide insertions/deletions (<15 bases). MutS alpha is associated with BLM:p53:RAD51 complexes, while MSH2 beta is associated with SLX4/BTBD12 complexes. Human MSH2 is 934 amino acids (aa) in length. It contains three MutS domains (aa 18-132; 158-284; 297-612) plus an ABC transporter signature motif that may hydrolyze ATP (aa 633-852). There are multiple splice variants. One shows a premature truncation after His429, while others contain a 46 aa substitution for aa 879-934, a 29 aa substitution for aa 462-934, a 28 aa substitution for aa 783-934, a 3 aa substitution for aa 532-934, a 5 aa substitution for aa 482‑934 and a 31 aa substitution for aa 379-934. Over aa 1-140, human MSH2 shares 94% aa identity with mouse MSH2.

Long Name
MutationS Homolog 2
Entrez Gene IDs
4436 (Human); 17685 (Mouse); 81709 (Rat)
Alternate Names
COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC; HNPCC1; HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1); LCFS2; MSH2; mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli); MutS protein homolog 2

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