Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 68801
View all MSP/MST1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MSP beta chain
Accession # P26927

Specificity

Detects recombinant human (rh)MSP in direct ELISAs and Western blots. When used in combination with the monoclonal capture antibody (Catalog # MAB352) in sandwich ELISAs, less than 0.001% cross-reactivity was observed with rhHGF, rhIL-9 R, rhMSP alpha or rmMSP R.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human MSP/MST1 beta Chain Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human MSP (Catalog # 352-MS)
under non-reducing conditions only

Human MSP Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human MSP alpha Chain Antibody (Catalog # MAB352)
  • Standard: Recombinant Human MSP/MST1 Protein (Catalog # 352-MS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSP/MST1

MSP, also known as hepatocyte growth factor-like protein (HGFl), and scatter factor-2 (SF2), is a member of the HGF family of growth factors. It is secreted as a single chain inactive precursor which is proteolytically activated to yield a biologically active disulfide-linked heterodimer of the alpha and beta chains. MSP induces keratinocyte proliferation, bone resorption, and cell migration and shape change in resident macrophages. Heterodimeric MSP binds to and activates the cell surface receptor tyrosine kinase, RON/STK, which is expressed on mature macrophages, keratinocytes, and vascular endothelium of wounded skin.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Alternate Names

HGFL, MST1, SF2

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Gene Symbol

MST1

UniProt

P26927

Additional MSP/MST1 Products

Product Documents for Human MSP/MST1 beta Chain Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MSP/MST1 beta Chain Biotinylated Antibody

For research use only

Related Research Areas

Inflammatory Mediators

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