Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Immunoaffinity Purification

Cited:

ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 45904
View all MSP/MST1 alpha Chain Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MSP
Met1-Gly711
Accession # AAA59872

Specificity

Detects human MSP alpha Chain in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) HGF or rhIL‑6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MSP/MST1 alpha Chain Antibody (45904)

Human MSP / MST1 ELISA Standard Curve

Human MSP / MST1 ELISA Standard Curve

Recombinant Human MSP/MST1 (Catalog # 352-MS) was serially diluted and captured by Mouse Anti-Human MSP/MST1 alpha Chain Monoclonal Antibody (Catalog # MAB352) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human MSP/MST1 beta Chain Monoclonal Antibody (Catalog # MAB735) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human MSP/MST1 alpha Chain Antibody (45904)

Application
Recommended Usage

Immunoaffinity Purification

Immobilized anti-MSP  alpha  chain antibody can be used to bind pro-MSP, mature MSP, and free alpha  chain.

Western Blot

1 µg/mL
Sample: Recombinant Human MSP (Catalog # 352-MS)
under non-reducing conditions only

Human MSP Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human MSP/MST1 beta Chain Biotinylated Antibody (Catalog # BAM3521)
  • Standard: Recombinant Human MSP/MST1 Protein (Catalog # 352-MS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB352 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSP/MST1 alpha Chain

MSP, also known as hepatocyte growth factor-like protein (HGFl), and scatter factor-2 (SF2), is a member of the HGF family of growth factors. It is secreted as a single chain inactive precursor which is proteolytically activated to yield a biologically active disulfide-linked heterodimer of the alpha and beta chains. MSP induces keratinocyte proliferation, bone resorption, and cell migration and shape change in resident macrophages. Heterodimeric MSP binds to and activates the cell surface receptor tyrosine kinase, RON/STK, which is expressed on mature macrophages, keratinocytes, and vascular endothelium of wounded skin.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Entrez Gene IDs

4485 (Human); 15325 (Mouse); 24566 (Rat)

Gene Symbol

MST1

UniProt

AAA59872 (Human)

Additional MSP/MST1 alpha Chain Products

Product Documents for Human MSP/MST1 alpha Chain Antibody (45904)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MSP/MST1 alpha Chain Antibody (45904)

For research use only

Citations for Human MSP/MST1 alpha Chain Antibody (45904)

Customer Reviews for Human MSP/MST1 alpha Chain Antibody (45904) (1)

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  • Human MSP alpha Chain Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 09/23/2019
    We used this antibody for developing a sandwich ELISA in combination with protein (352-MS) and pAb (AF352). This combination worked well and detected human MSP in serum samples efficiently.
    Human MSP/MST1 alpha Chain Antibody (45904) MAB352

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