Human MSP alpha Chain Antibody

Catalog # Availability Size / Price Qty
MAB352
MAB352-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human MSP alpha Chain Antibody Summary

Species Reactivity
Human
Specificity
Detects human MSP alpha Chain in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) HGF or rhIL‑6 is observed.
Source
Monoclonal Mouse IgG1 Clone # 45904
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP
Met1-Gly711
Accession # AAA59872
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MSP (Catalog # 352-MS)
under non-reducing conditions only
Immunoaffinity Purification
Immobilized anti-MSP  alpha  chain antibody can be used to bind pro-MSP, mature MSP, and free alpha  chain.
 

Human MSP Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human MSP/MST1 beta Chain Biotinylated Antibody (Catalog # BAM3521)

Standard: Recombinant Human MSP/MST1 Protein (Catalog # 352-MS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP/MST1

MSP, also known as hepatocyte growth factor-like protein (HGFl), and scatter factor-2 (SF2), is a member of the HGF family of growth factors. It is secreted as a single chain inactive precursor which is proteolytically activated to yield a biologically active disulfide-linked heterodimer of the alpha and beta chains. MSP induces keratinocyte proliferation, bone resorption, and cell migration and shape change in resident macrophages. Heterodimeric MSP binds to and activates the cell surface receptor tyrosine kinase, RON/STK, which is expressed on mature macrophages, keratinocytes, and vascular endothelium of wounded skin.

Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Entrez Gene IDs
4485 (Human); 15235 (Mouse); 24566 (Rat)
Alternate Names
D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2

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Citations for Human MSP alpha Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. HIV?1 downregulates the expression and phosphorylation of receptor tyrosine kinase by targeting the NF??B pathway
    Mol Med Rep, 2016;0(0):.
    Species: Human
    Sample Types: Whole Blood
    Applications: ELISA Development (Capture)
  2. Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele.
    Authors: Kauder, Steven E, Santell, Lydia, Mai, Elaine, Wright, Lilyan Y, Luis, Elizabet, N'Diaye, Elsa N, Lutman, Jeff, Ratti, Navneet, Sa, Susan M, Maun, Henry R, Stefanich, Eric, Gonzalez, Lino C, Graham, Robert R, Diehl, Lauri, Faubion, William, Keir, Mary E, Young, Judy, Chaudhuri, Amitabha, Lazarus, Robert A, Egen, Jackson
    PLoS ONE, 2013;8(12):e83958.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MSP alpha Chain Antibody

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Human MSP alpha Chain Antibody
By Anonymous on 09/23/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody for developing a sandwich ELISA in combination with protein (352-MS) and pAb (AF352). This combination worked well and detected human MSP in serum samples efficiently.