Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 68801
View all MSP/MST1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MSP beta chain
Val484-Gly711
Accession # P26927

Specificity

Detects recombinant human (rh)MSP beta chain in direct ELISAs and Western blots. Shows no cross-reactivity with rhMSP alpha  chain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human MSP beta Chain Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.01-0.05 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human MSP (Catalog # 4306-MS) to immobilized Recombinant Human MSP R (Catalog # 1947-MS) coated at 1 μg/mL (100 µL/well). At 1 µg/mL, this antibody will block >90% of the binding.

Western Blot

1 µg/mL
Sample: Recombinant Human MSP  beta Chain (Catalog # 635-MS) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSP/MST1

MSP, also known as hepatocyte growth factor-like protein (HGFl), and scatter factor-2 (SF2), is a member of the HGF family of growth factors. It is secreted as a single chain inactive precursor which is proteolytically activated to yield a biologically active disulfide-linked heterodimer of the alpha and beta chains. MSP induces keratinocyte proliferation, bone resorption, and cell migration and shape change in resident macrophages. Heterodimeric MSP binds to and activates the cell surface receptor tyrosine kinase, RON/STK, which is expressed on mature macrophages, keratinocytes, and vascular endothelium of wounded skin.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Alternate Names

HGFL, MST1, SF2

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Gene Symbol

MST1

UniProt

P26927

Additional MSP/MST1 Products

Product Documents for Human MSP beta Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MSP beta Chain Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Human MSP beta Chain Antibody

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