Detection of MSP R/Ron in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human MSP R/Ron PerCP‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6647C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: MSP R/Ron
RON (Recepteur d'Origine Nantais [Receptor originating (or
discovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a
180-190 kDa member of the tyrosine kinase family, protein kinase superfamily of
molecules. Human mature RON is a type I
transmembrane heterodimeric glycoprotein that arises from the proteolytic cleavage
of a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a
35-40 kDa a-chain
(aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and the
transmembrane segment appears over aa 958-978.
Multiple cell types are known to express RON, and these include
osteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized and
nonkeratinized stratified squamous epithelium, intestinal Paneth cells, renal
proximal tubule and mesangial cells, monocytes, neutrophils and mammary
epithelium. On the cell surface, RON
serves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization and
receptor signaling. In addition, RON is
also known to heterodimerize with multiple integral membrane proteins, either
individually or as part of a complex.
Proteins identified to date include b1 integrins, c-MET/HGFR,
E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, and
CD44v6. RON may also be found
intracellularly, where it interacts with either the androgen receptor or EGFR
in the cytoplasm, or in the nucleus, where it acts as a transcription factor
coupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There are
about a dozen alternative splice forms for RON, some soluble, while others are
transmembrane, and either constitutively active or biochemically inert. They
hold the potential to interact with full-length RON and impact its signaling
capabilities. Reflecting the diverse
nature of RON-expressing cells, RON has been found to generate multiple
outcomes, including the promotion of epithelial-to-mesenchymal transitions, the
maintenance of M2 macrophage phenotypes, and the initiation of keratinocyte
proliferation and migration. Over aa
25-956, human and mouse RON share 76% aa sequence identity.
Macrophage Stimulating Protein Receptor
Entrez Gene IDs:
4486 (Human); 19882 (Mouse)
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 220.127.116.11; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4
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