Human MTAP Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB11747AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 1113601
View all MTAP Primary Antibodies »

Product Specifications

Specificity

Detects recombinant human MTAP protein in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human MTAP Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MTAP

Methyl-thioadenosine phosphorylase/MTAP is part of the PNP/MTAP phosphorylase family and catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA), a major byproduct of polyamine synthesis essential for cell growth and proliferation. MTAP also produces most of the free adenine generated in human cells through a salvage pathway and thus couples the purine salvage pathway with polyamine biosynthesis. MTAP forms an active trimer where each identical 32 kDa monomer contains a separate active site (1). Each active site contains three distinct regions required for base-, methylthioribose-, and sulfate/phosphate-binding (1). MTAP is cytosolic and abundantly expressed in normal cells and tissues (2). In contrast, deficient MTAP expression is observed in many types of tumors including lung, bladder, pancreatic, and endometrial cancer (3) due to hyper-methylation gene suppression (4) or gene deletion (3, 5, 6). MTA accumulation leads to an immunosuppressive tumor microenvironment and apoptotic resistance (7-9) and MTAP directly regulates the level of MTA present. MTAP has been reported as a tumor suppressor (6,10) that may also act in a manner that is independent of enzymatic activity (11) through signaling pathways such as the insulin-like growth factor-1 receptor pathway (12). Potential therapeutic strategies to exploit MTAP deficiency in tumors (13,14) or inhibit MTAP in tumors that express MTAP, such as prostate cancer, are under investigation (15).

References

  1. Appleby, T.C. et al. (1999) Structure 7:629.
  2. Garbers, D.L. (1978) Biochim. Biophys. Acta. 523:82.
  3. Li, Y. et al. (2019) J. Cancer 10:927.
  4. Hellerbrand, C. et al. (2006) Carcinogenesis 27:64.
  5. Cairns, P. et al. (1995) Nat. Genet. 11:210.
  6. Christopher, S.A. (2002) Cancer Res. 62:6639.
  7. Kirovski, G. et al. (2011) Am. J. Pathol. 178:1145.
  8. Czech, B. et al. (2013) PLoS One 8:e80703.
  9. Henrich, F.C. et al. (2016) Oncoimmunology 5:e1184802.
  10. Kadariya, Y. et al. (2009) Cancer Res. 69:5961.
  11. Tang, B. et al. (2014) G3 (Bethesda) 5:35.
  12. Xu, J. et al. (2019) Signal Transduct. Target Ther. 4:2.
  13. Bertino, J.R. et al. (2011) Cancer Biol. Ther. 11:627.
  14. Tang, B. et al. (2018) Cancer Res. 78:4386.
  15. Bistulfi, G. et al. (2016) Oncotarget 7:14380.

Long Name

5'-Methylthioadenosine Phosphorylase

Alternate Names

BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase

Entrez Gene IDs

4507 (Human); 66902 (Mouse)

Gene Symbol

MTAP

UniProt

Q13126 (Human)

Additional MTAP Products

Product Documents for Human MTAP Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MTAP Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Sulfation Regulators

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Protocols

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FAQs

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